비스페놀A 탐지를 위한 DNAzyme기반의 압타센서 연구

Abstract

Bisphenol A (BPA) belongs to endocrine disrupting chemicals or endocrine disruptors (EDCs), which have been suspected to be associated with reproductive dysfunction and physiological abnormality in human. Since the BPA has been widely used to make plastics and epoxy resins, the leach of BPA from the lining of plastic products has been of major concern, due to its environmental or human exposure issues. However, despite the growing advance in sensing technology, most of attempts to sense the BPA have relied upon the traditional methods including liquid chromatography (LC) and enzyme-linked immunosorbent assay (ELISA), which are labor-intensive and/or low-throughput. In the present study, DNA-based biosensors, consisting of DNAzyme and DNA aptamer, have been developed for the rapid and simple detection of BPA. DNAzyme-based aptasensors include a sensing part (anti-BPA aptamer) and a signal generation part (horseradish peroxides-mimicking DNAzyme induced by G-quadruplex structure in the presence of hemin). Two types of BPA aptasensors were designed based on i) split-DNAzyme or ii) signal amplification by hybridization of chain reaction (HCR) including the full-length DNAzyme sequence. The split-DNAzyme-based aptasensors detected the BPA as low as 20 nM, whereas the HCR-type aptasensors showed an increased performance which has potency to detect the BPA lower than 2 nM. In addition, both types of aptasensors exhibited high selectivity toward BPA over other EDCs. In this regard, it is anticipated that the designed DNAzyme-based aptasensors will find many applications for the detection of EDCs with high sensitivity and selectivity in a rapid format.