인간 배아줄기세포에서 증식 가능한 중뇌 신경줄기세포로 분화유도: 중뇌 도파민신경세포 관련된 질병 연구를 위한 세포 시스템

Abstract

Midbrain-type DA (mDA) neurons made by directed differentiation of hESCs provide disease modeling related to mDA neuron, and systematic cell source for cell transplantation. By the way, to make the systematic cell source, it is required that the cells pass by the neural stem cell stage. Also, it is important that the mDA neuron which is differentiated from the hESC expressing the midbrain marker. Because midbrain markers are required to maintain the mDA neuron phenotype, function, survival. Therefore, I treated several combinations of the chemical that inhibit/activate the signaling related to the development of the mDA neuron base on the nature protocol and used the mitogen to make the cell proliferate. After that I can observe the markers specific for midbrain cells, neural stem cells, mDA neurons. Here, I suggest the new protocol for generating the expandable NSC expressing Foxa2 and Lmx1a and resultantly generating mDA neuron.