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Development of Line Scan Confocal Raman Microscopy using a Spectrometer with Improved Diffraction Efficienc

Title
Development of Line Scan Confocal Raman Microscopy using a Spectrometer with Improved Diffraction Efficienc
Author
형경초
Alternative Author(s)
형경초
Advisor(s)
이종민
Issue Date
2020-02
Publisher
한양대학교
Degree
Doctor
Abstract
Confocal Raman microscopy is widely used in various fields of research since it allows for label-free sample investigation. However, the slow data acquisition speed of Raman microscopy limits the applications for imaging large area samples. I developed a line-scan confocal Raman microscope for high-speed data acquisition and proposed a dual mirror scan method to improve the diffraction efficiency of the spectrometer. I used a Powell lens for the line illumination and demonstrated that it can provide a more uniform intensity distribution than a cylindrical lens. A volume phase holographic grating was used in the spectrometer due to it can provide higher diffraction efficiency than the surface-relief grating. The proposed dual mirror scan method can provide a high-resolution Raman spectrum at high efficiency over a wide spectral range. By combining the system with a high sensitivity EMCCD camera, the maximum spectral rate can reach to 25,000 spectra/s. I demonstrated the performance by imaging a custom-designed large area sample and arterial tissue sections. With the parallel detection by the line excitation and the improved diffraction efficiency, I expect the proposed system can be used in a variety of applications that require a high spectral rate.
URI
https://repository.hanyang.ac.kr/handle/20.500.11754/123468http://hanyang.dcollection.net/common/orgView/200000436803
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIOMEDICAL ENGINEERING(생체공학과) > Theses (Ph.D.)
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