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dc.contributor.author정승준-
dc.date.accessioned2019-12-10T15:47:02Z-
dc.date.available2019-12-10T15:47:02Z-
dc.date.issued2018-12-
dc.identifier.citationBIOSENSORS & BIOELECTRONICS, v. 121, page. 174-182en_US
dc.identifier.issn0956-5663-
dc.identifier.issn1873-4235-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S095656631830633X?via%3Dihub-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/121002-
dc.description.abstractFor over two decades, fast-scan cyclic voltammetry (FSCV) has served as a reliable analytical method for monitoring dopamine release in near real-time in vivo. However, contemporary FSCV techniques have been limited to measure only rapid (on the order of seconds, i.e. phasic) changes in dopamine release evoked by either electrical stimulation or elicited by presentation of behaviorally salient stimuli, and not slower changes in the tonic extracellular levels of dopamine (i.e. basal concentrations). This is because FSCV is inherently a differential method that requires subtraction of prestimulation tonic levels of dopamine to measure phasic changes relative to a zeroed baseline. Here, we describe the development and application of a novel voltammetric technique, multiple cyclic square wave voltammetry (M-CSWV), for analytical quantification of tonic dopamine concentrations in vivo with relatively high temporal resolution (10 s). M-CSWV enriches the electrochemical information by generating two dimensional voltammograms which enable high sensitivity (limit of detection, 0.17 nM) and selectivity against ascorbic acid, and 3,4-dihydroxyphenylacetic acid (DOPAC), including changes in pH. Using M-CSWV, a tonic dopamine concentration of 120 +/- 18 nM (n = 7 rats, +/- SEM) was determined in the striatum of urethane anethetized rats. Pharmacological treatments to elevate dopamine by selectively inhibiting dopamine reuptake and to reduce DOPAC by inhibition of monoamine oxidase supported the selective detection of dopamine in vivo. Overall, M-CSWV offers a novel voltammetric technique to quantify levels and monitor changes in tonic dopamine concentrations in the brain to further our understanding of the role of dopamine in normal behavior and neuropsychiatric disorders.en_US
dc.description.sponsorshipThis research was supported by the NIH 1U01NS090455-01 award and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2017R1A2B2006896).en_US
dc.language.isoen_USen_US
dc.publisherELSEVIER ADVANCED TECHNOLOGYen_US
dc.subjectElectrochemistryen_US
dc.subjectDopamineen_US
dc.subjectCarbon-fiber microelectrodesen_US
dc.subjectStriatumen_US
dc.subjectRaten_US
dc.titleTracking tonic dopamine levels in vivo using multiple cyclic square wave voltammetryen_US
dc.typeArticleen_US
dc.relation.volume121-
dc.identifier.doi10.1016/j.bios.2018.08.034-
dc.relation.page174-182-
dc.relation.journalBIOSENSORS & BIOELECTRONICS-
dc.contributor.googleauthorOh, Yoonbae-
dc.contributor.googleauthorHeien, Michael L.-
dc.contributor.googleauthorPark, Cheonho-
dc.contributor.googleauthorKang, Yu Min-
dc.contributor.googleauthorKim, Jaekyung-
dc.contributor.googleauthorBoschen, Suelen Lucio-
dc.contributor.googleauthorShin, Hojin-
dc.contributor.googleauthorCho, Hyun U.-
dc.contributor.googleauthorBlaha, Charles D.-
dc.contributor.googleauthorJung, Sung Jun-
dc.relation.code2018001457-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pideurijj-
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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