Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2019-12-10T04:37:40Z | - |
dc.date.available | 2019-12-10T04:37:40Z | - |
dc.date.issued | 2018-11 | - |
dc.identifier.citation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 410, no. 27, page. 7019-7030 | en_US |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.issn | 1618-2650 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs00216-018-1335-9 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/120701 | - |
dc.description.abstract | A variety of automated sample-in-answer-out systems for in vitro molecular diagnostics have been presented and even commercialized. Although efficient in operation, they are incapable of quantifying targets, since quantitation based on analog analytical methods (via standard curve analysis) is complex, expensive, and challenging. To address this issue, herein, we describe an integrated sample-in-digital-answer-out (SIDAO) diagnostic system incorporating DNA extraction and digital recombinase polymerase amplification, which enables rapid and quantitative nucleic acid analysis from bodily fluids within a disposable cartridge. Inside the cartridge, reagents are pre-stored in sterilized tubes, with an automated pipetting module allowing facile liquid transfer. For digital analysis, we fabricate a simple, single-layer polydimethylsiloxane microfluidic device and develop a novel and simple sample compartmentalization strategy. Sample solution is partitioned into an array of 40,044 fL-volume microwells by sealing the microfluidic device through the application of mechanical pressure. The entire analysis is performed in a portable, fully automated instrument. We evaluate the quantitative capabilities of the system by analyzing Mycobacterium tuberculosis genomic DNA from both spiked saliva and serum samples, and demonstrate excellent analytical accuracy and specificity. This SIDAO system provides a promising diagnostic platform for quantitative nucleic acid testing at the point-of-care. | en_US |
dc.description.sponsorship | This work was supported by the Swiss Federal Institute of Technology (ETH Zurich), the National Research Foundation of Korea (Grant Nos. 2008-0061891 and 2009-00426), the National Natural Science Foundation of China (Grant No. 61527806), the Hunan Key Research Project (Grant No. 2017SK2174), and the China Postdoctoral Science Foundation (Grant No. 2018M630498). P.D.H. acknowledges support from European Union's Horizon 2020 research and innovation program through the Individual Marie Skodowska-Curie Fellowship "Ampidots" under grant agreement no. 701994. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | SPRINGER HEIDELBERG | en_US |
dc.subject | Sample-in-answer-out | en_US |
dc.subject | Microfluidics | en_US |
dc.subject | Quantitation | en_US |
dc.subject | Digital RPA | en_US |
dc.subject | Molecular diagnostics | en_US |
dc.subject | Tuberculosis | en_US |
dc.title | A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids | en_US |
dc.type | Article | en_US |
dc.relation.no | 27 | - |
dc.relation.volume | 410 | - |
dc.identifier.doi | 10.1007/s00216-018-1335-9 | - |
dc.relation.page | 7019-7030 | - |
dc.relation.journal | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.contributor.googleauthor | Yang, Haowen | - |
dc.contributor.googleauthor | Chen, Zhu | - |
dc.contributor.googleauthor | Cao, Xiaobao | - |
dc.contributor.googleauthor | Li, Zhiyang | - |
dc.contributor.googleauthor | Stavrakis, Stavros | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.contributor.googleauthor | deMello, Andrew J. | - |
dc.contributor.googleauthor | Howes, Philip D. | - |
dc.contributor.googleauthor | He, Nongyue | - |
dc.relation.code | 2018000979 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.