Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박장환 | - |
dc.date.accessioned | 2019-12-10T01:33:38Z | - |
dc.date.available | 2019-12-10T01:33:38Z | - |
dc.date.issued | 2018-11 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v. 8, Article no. 16721 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.nature.com/articles/s41598-018-35077-0 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/120585 | - |
dc.description.abstract | Abnormalities in gene expression that negatively affect embryonic development are frequently observed in cloned embryos generated by somatic cell nuclear transfer (SCNT). In the present study, we successfully produced a cell-penetrating peptide (CPP)-conjugated with coactivator-associated arginine methyltransferase 1 (CARM1) protein from mammalian cells and confirmed introduction into donor somatic cells and cloned 8-cell embryos within 3 hours after addition to culture medium. In addition, H3R17 dimethylation and embryonic development up to the blastocyst stage were increased in the group treated with exogenous CPP-CARM1 protein compared with the untreated group (control). Interestingly, the number of total cells and trophectoderm in blastocysts as well as implantation rate were significantly increased in the CPP-CARM1 protein-treated group. However, the cell number of inner cell mass (ICM) was not changed compared with the control group; similarly, expression of pluripotency-related genes Oct4 and Nanog (ICM markers) was not significantly different between groups. On the other hand, expression of the implantation-related gene Cdx2 (trophectoderm marker) was transiently increased after treatment with CPP-CARM1 protein. On the basis of these results, we conclude that supplementation with exogenous CPP-CARM1 protein improves embryonic development of cloned embryos through regulation of histone methylation and gene expression. In addition, our results suggest that CPP-CARM1 protein may be a useful tool for strengthening implantation of mammalian embryos. | en_US |
dc.description.sponsorship | This research was supported partly by the Bio & Medical Technology Development Program (2015M3A9C6028961, 2017M3A9C8029318 and 2017M3A9C6061284) and Basic Science Research (2009-0093821) of the National Research Foundation (NRF) of Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | NUCLEAR TRANSFER | en_US |
dc.subject | STEM-CELLS | en_US |
dc.subject | CLONING EFFICIENCY | en_US |
dc.subject | SOMATIC-CELLS | en_US |
dc.subject | METHYLATION | en_US |
dc.subject | IMPROVES | en_US |
dc.subject | ADULT | en_US |
dc.subject | DELIVERY | en_US |
dc.subject | MICE | en_US |
dc.title | The effect of cell penetrating peptide-conjugated coactivator-associated arginine methyltransferase 1 (CPP-CARM1) on the cloned mouse embryonic development | en_US |
dc.type | Article | en_US |
dc.relation.volume | 8 | - |
dc.identifier.doi | 10.1038/s41598-018-35077-0 | - |
dc.relation.page | 16721-16729 | - |
dc.relation.journal | SCIENTIFIC REPORTS | - |
dc.contributor.googleauthor | Bang, Jae-Il | - |
dc.contributor.googleauthor | Lee, Eun-Hye | - |
dc.contributor.googleauthor | Lee, Ah Reum | - |
dc.contributor.googleauthor | Lee, Jin Il | - |
dc.contributor.googleauthor | Choi, Seo Hye | - |
dc.contributor.googleauthor | Seol, Dong-Won | - |
dc.contributor.googleauthor | Park, Chang-Hwan | - |
dc.contributor.googleauthor | Lee, Dong Ryul | - |
dc.relation.code | 2018003596 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | chshpark | - |
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