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Highly sensitive detection of high-risk bacterial pathogens using SERS-based lateral flow assay strips

Title
Highly sensitive detection of high-risk bacterial pathogens using SERS-based lateral flow assay strips
Author
주재범
Keywords
Yersinia pestis; Francisella tularensis; Bacillus anthraces; Surface enhanced Raman scattering; Lateral flow assay
Issue Date
2018-10
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v. 270, page. 72-79
Abstract
Bacterial pathogens such as Yersinia pestis, Francisella tularensis, and Bacillus anthraces are classified into the highest rank of potential bioterrorism agents. Colorimetric lateral flow assay (LFA) strips are commercially available but these conventional strips have drawbacks in terms of low sensitivity and limit of quantitative analysis. Therefore, there is an urgent need for a new sensing platform to detect these pathogens in the early contamination stage. In this study, a novel surface-enhanced Raman scattering (SERS)-based LFA strip was developed for sensitive detection of bacterial pathogens. Target-specific SERS nanotags (Raman reporter-labeled gold nanoparticles) were used as an alternative to the gold nanoparticles in conventional LFA strips. Using these SERS nanotags the presence of bacteria could be identified through a simple color change in the test line. Additionally, highly sensitive and accurate quantitative analysis could be performed by monitoring the characteristic Raman peak intensity of SERS nanotags that were captured in the test line. This highly sensitive method required a short assay time (15 min) and a tiny volume of pathogen sample (40 mu L). We believe that the proposed SERS-based LFA technique has great potential as a valuable tool in the early detection of specific bacterial pathogens in the field due to its excellent analytical sensitivity. (C) 2018 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0925400518308736?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/120390
ISSN
0925-4005
DOI
10.1016/j.snb.2018.04.162
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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