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Reprogramming mechanisms influence the maturation of hematopoietic progenitors from human pluripotent stem cells

Title
Reprogramming mechanisms influence the maturation of hematopoietic progenitors from human pluripotent stem cells
Author
김계성
Keywords
NUCLEAR TRANSFER; DEFINED FACTORS; DOWN-REGULATION; DIFFERENTIATION; EXPRESSION; CULTURES; LINES
Issue Date
2018-10
Publisher
NATURE PUBLISHING GROUP
Citation
CELL DEATH & DISEASE, v. 9, Article no. 1090
Abstract
Somatic cell nuclear transfer (SCNT) or the forced expression of transcription factors can be used to generate autologous pluripotent stem cells (PSCs). Although transcriptomic and epigenomic comparisons of isogenic human NT-embryonic stem cells (NT-ESCs) and induced PSCs (iPSCs) in the undifferentiated state have been reported, their functional similarities and differentiation potentials have not been fully elucidated. Our study showed that NT-ESCs and iPSCs derived from the same donors generally displayed similar in vitro commitment capacity toward three germ layer lineages as well as proliferative activity and clonogenic capacity. However, the maturation capacity of NT-ESC-derived hematopoietic progenitors was significantly greater than the corresponding capacity of isogenic iPSC-derived progenitors. Additionally, donor-dependent variations in hematopoietic specification and commitment capacity were observed. Transcriptome and methylome analyses in undifferentiated NT-ESCs and iPSCs revealed a set of genes that may influence variations in hematopoietic commitment and maturation between PSC lines derived using different reprogramming methods. Here, we suggest that genetically identical iPSCs and NT-ESCs could be functionally unequal due to differential transcription and methylation levels acquired during reprogramming. Our proof-of-concept study indicates that reprogramming mechanisms and genetic background could contribute to diverse functionalities between PSCs.
URI
https://www.nature.com/articles/s41419-018-1124-6https://repository.hanyang.ac.kr/handle/20.500.11754/120255
ISSN
2041-4889
DOI
10.1038/s41419-018-1124-6
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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