Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김용희 | - |
dc.date.accessioned | 2019-12-08T17:56:41Z | - |
dc.date.available | 2019-12-08T17:56:41Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, v. 50, Article no. 105 | en_US |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://www.nature.com/articles/s12276-018-0124-z | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119518 | - |
dc.description.abstract | An excess of reactive oxygen species (ROS) relative to the antioxidant capacity causes oxidative stress, which plays a role in the development of Parkinson's disease (PD). Because mitochondria are both sites of ROS generation and targets of ROS damage, the delivery of antioxidants to mitochondria might prevent or alleviate PD. To transduce the antioxidant protein human metallothionein 1A (hMT1A) into mitochondria, we computationally designed a cell-penetrating artificial mitochondria-targeting peptide (CAMP). The recombinant CAMP-conjugated hMT1A fusion protein (CAMP-hMT1A) successfully localized to the mitochondria. Treating a cell culture model of PD with CAMP-hMT1A restored tyrosine hydroxylase expression and mitochondrial activity and reduced ROS production. Furthermore, injection of CAMP-hMT1A into the brain of a mouse model of PD rescued movement impairment and dopaminergic neuronal degeneration. CAMP-hMT1A delivery into mitochondria might be therapeutic against PD by alleviating mitochondrial damage, and we predict that CAMP could be used to deliver other cargo proteins to the mitochondria. | en_US |
dc.description.sponsorship | This research was supported by grant HI14C2700 from the Korean Health Technology R&D Project and by the Technology Innovation Program (10051960 and 10051188) from the Ministry of Trade, Industry & Energy, South Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | PROTEIN TRANSDUCTION | en_US |
dc.subject | ENHANCED DELIVERY | en_US |
dc.subject | OXIDATIVE STRESS | en_US |
dc.subject | PATHOGENESIS | en_US |
dc.subject | DYSFUNCTION | en_US |
dc.subject | MECHANISMS | en_US |
dc.subject | COMPLEX | en_US |
dc.subject | IMPORT | en_US |
dc.title | Cell-penetrating artificial mitochondria-targeting peptide-conjugated metallothionein 1A alleviates mitochondrial damage in Parkinson's disease models | en_US |
dc.type | Article | en_US |
dc.relation.volume | 50 | - |
dc.identifier.doi | 10.1038/s12276-018-0124-z | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Kang, Young Cheol | - |
dc.contributor.googleauthor | Son, Minuk | - |
dc.contributor.googleauthor | Kang, Sora | - |
dc.contributor.googleauthor | Im, Suyeol | - |
dc.contributor.googleauthor | Piao, Ying | - |
dc.contributor.googleauthor | Lim, Kwang Suk | - |
dc.contributor.googleauthor | Song, Min-Young | - |
dc.contributor.googleauthor | Park, Kang-Sik | - |
dc.contributor.googleauthor | Kim, Yong-Hee | - |
dc.contributor.googleauthor | Pak, Youngmi Kim | - |
dc.relation.code | 2018002394 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | yongheekim | - |
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