Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤지희 | - |
dc.date.accessioned | 2019-12-08T17:28:12Z | - |
dc.date.available | 2019-12-08T17:28:12Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | FRONTIERS IN IMMUNOLOGY, v. 9, Article no. 1544 | en_US |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fimmu.2018.01544/full | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119499 | - |
dc.description.abstract | Objective: To gain a better understanding of the pathogenesis of autoimmune arthritis-associated interstitial lung disease (ILD), we sought to identify the characteristics of lung-infiltrating cells in SKG mice with ILD.Methods: We injected curdlan in SKG mice at 8 weeks of age, and identified the presence of ILD by PET-MRI at 20 weeks post-injection and histological analysis at 22 weeks post-injection. Lung-infiltrating cells were examined by flow cytometry. Analysis of serum cytokines by the Luminex multiplex cytokine assay was performed at 14 and 22 weeks post-injection, and cytokine profiles before and after the development of ILD were compared. Opal multiplexed immunofluorescent staining of lung tissue was also performed.Results: At 20 weeks post-injection, curdlan-treated SKG mice developed not only arthritis but also lung inflammation combined with fibrosis, which was identified by PET-MRI and histological analysis. The majority of inflammatory cells that accumulated in the lungs of curdlan-treated SKG mice were CD11b(+)Gr1(+) neutrophils, which co-express IL-17A and GM-CSF, rather than TNF-alpha. Compared with 14 weeks post-injection, serum levels of GM-CSF, MCP1, IL-17A, IL-23, TSLP, and soluble IL-7R alpha had increased at 22 weeks post-injection, whereas those of IFN-gamma, IL-22, IL-6, and TNF-alpha remained unchanged. Furthermore, IL-23, CXCL5, IL-17A, and GM-CSF, but not TNF-alpha, were observed in immunofluorescent-stained lung tissue.Conclusion: We found that IL-17A(+)GM-CSF+ neutrophils represented the major inflammatory cells in the lungs of curdlan-treated SKG mice. In addition, GM-CSF and IL-17A appear to play a more important role than TNF-alpha in ILD development. | en_US |
dc.description.sponsorship | This work was supported by grants from the National Research Foundation of Korea to Y-GK (2016R1D1A1A02936963). We would like to thank the PET and the optical imaging core facility at the ConveRgence mEDIcine research center (CREDIT) at Asan Medical Center for technical support. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | FRONTIERS MEDIA SA | en_US |
dc.subject | GM-CSF | en_US |
dc.subject | IL-17A | en_US |
dc.subject | neutrophil | en_US |
dc.subject | autoimmune arthritis | en_US |
dc.subject | interstitial lung disease | en_US |
dc.title | IL-17A(+)GM-CSF+ Neutrophils Are the Major Infiltrating Cells in Interstitial Lung Disease in an Autoimmune Arthritis Model | en_US |
dc.type | Article | en_US |
dc.relation.volume | 9 | - |
dc.identifier.doi | 10.3389/fimmu.2018.01544 | - |
dc.relation.page | 1-9 | - |
dc.relation.journal | FRONTIERS IN IMMUNOLOGY | - |
dc.contributor.googleauthor | Kwon, Oh Chan | - |
dc.contributor.googleauthor | Lee, Eun-Ju | - |
dc.contributor.googleauthor | Chang, Eun-Ju | - |
dc.contributor.googleauthor | Youn, Jeehee | - |
dc.contributor.googleauthor | Ghang, Byeongzu | - |
dc.contributor.googleauthor | Hong, Seokchan | - |
dc.contributor.googleauthor | Lee, Chang-Keun | - |
dc.contributor.googleauthor | Yoo, Bin | - |
dc.contributor.googleauthor | Kim, Yong-Gil | - |
dc.relation.code | 2018006826 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jhyoun | - |
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