Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한중수 | - |
dc.date.accessioned | 2019-12-08T14:57:21Z | - |
dc.date.available | 2019-12-08T14:57:21Z | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | MOLECULAR BRAIN, v. 11, Article no. 39 | en_US |
dc.identifier.issn | 1756-6606 | - |
dc.identifier.uri | https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-018-0383-6 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119435 | - |
dc.description.abstract | Pro-inflammatory cytokine interleukin-1 beta (IL-1 beta) is a key mediator of inflammation and stress in the central nervous system (CNS), and is highly expressed in the developing brain. In this study, we investigated the possible role of IL-1 beta in neuronal differentiation of cortical neural precursor cells (NPCs). We showed that stimulation with IL-1 beta increased expression levels of neurotrophin-3 (NT3) and neurogenin 1 (Ngn1) and promoted neurite outgrowth. We also found that IL-1 beta increased mRNA and protein levels of Wnt5a. Knockdown of Wnt5a by transfection with Wnt5a siRNA inhibited IL-1 beta-induced neuronal differentiation. Moreover, IL-1 beta-induced Wnt5a expression was regulated by nuclear factor kappa B (NT-kappa B) activation, which is involved in IL-1 beta-mediated neuronal differentiation. To examine the role of Wnt5a in neuronal differentiation of NPCs, we exogenously added Wnt5a. We found that exogenous Wnt5a promotes neuronal differentiation, and activates the RhoA/Rho-associated kinase (ROCK)/c-jun N-terminai kinase (JNK) pathway. In addition, Wnt5a-induced neuronal differentiation was blocked by RhoA siRNA, as well as by a specific Rho-kinase inhibitor (Y27632) or a SAPK/JNK inhibitor (SP600125). Furthermore, treatment with RhoA siRNA, Y27632, or SP600125 suppressed the IL-1 beta-induced neuronal differentiation. Therefore, these results suggest that the sequential Wnt5a/RhoA/ROCK/JNK pathway is involved in IL-1 beta-induced neuronal differentiation of NPCs. | en_US |
dc.description.sponsorship | This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, & Future Planning (NRF-2015R1C1A1A02037376), and partly supported by (NRF-2018R1A1A1A05022185). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BMC | en_US |
dc.subject | Interleukin-1 beta (IL-1 beta) | en_US |
dc.subject | Neuronal differentiation | en_US |
dc.subject | Wnt5a | en_US |
dc.subject | RhoA | en_US |
dc.subject | C-jun N-terminal kinase (JNK) | en_US |
dc.title | Interleukin-1 beta promotes neuronal differentiation through the Wnt5a/RhoA/ JNK pathway in cortical neural precursor cells | en_US |
dc.type | Article | en_US |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.1186/s13041-018-0383-6 | - |
dc.relation.page | 39-50 | - |
dc.relation.journal | MOLECULAR BRAIN | - |
dc.contributor.googleauthor | Park, Shin-Young | - |
dc.contributor.googleauthor | Kang, Min-Jeong | - |
dc.contributor.googleauthor | Han, Joong-Soo | - |
dc.relation.code | 2018006268 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jshan | - |
dc.identifier.researcherID | P-2072-2015 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-0875-6158 | - |
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