301 0

Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions

Title
Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions
Author
신흥수
Keywords
NF-spheroids; Hybrid spheroids; Composite spheroid; 3D culture; Polydopamine; ECM
Issue Date
2018-06
Publisher
ELSEVIER SCIENCE INC
Citation
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v. 67, page. 80-91
Abstract
Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.
URI
https://www.sciencedirect.com/science/article/pii/S1226086X18303071?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/119187
ISSN
1226-086X; 1876-794X
DOI
10.1016/j.jiec.2018.06.017
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE