Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions
- Title
- Agglomeration of human dermal fibroblasts with ECM mimicking nano-fragments and their effects on proliferation and cell/ECM interactions
- Author
- 신흥수
- Keywords
- NF-spheroids; Hybrid spheroids; Composite spheroid; 3D culture; Polydopamine; ECM
- Issue Date
- 2018-06
- Publisher
- ELSEVIER SCIENCE INC
- Citation
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v. 67, page. 80-91
- Abstract
- Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.
- URI
- https://www.sciencedirect.com/science/article/pii/S1226086X18303071?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/119187
- ISSN
- 1226-086X; 1876-794X
- DOI
- 10.1016/j.jiec.2018.06.017
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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