Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2019-12-08T11:34:55Z | - |
dc.date.available | 2019-12-08T11:34:55Z | - |
dc.date.issued | 2018-06 | - |
dc.identifier.citation | ANALYST, v. 143, no. 11, page. 2604-2615 | en_US |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.issn | 1364-5528 | - |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlelanding/2018/AN/C8AN00329G#!divAbstract | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/119155 | - |
dc.description.abstract | Surface-enhanced Raman scattering (SERS) is an optical spectroscopy technique that can detect a variety of analytes with high sensitivity and selectivity without any labels. Controlled clustering of metallic nanoparticles to prepare a new class of SERS nanotags is crucial for the ultra-sensitive detection of specific biological and chemical moieties because increased plasmonic hotspot junctions produce a greatly enhanced SERS signal. We report herein that controlled clustering of Au nanoparticles (AuNPs) was mediated by PEGylated nano-sized graphene (PNG) and that the PNG-induced AuNP clusters (PNG-AuNPCs) were highly sensitive SERS nanotags with colloidal stability for SERS-based biosensing. The AuNPs labeled with 4-mercaptopyridine as a Raman reporter were surface-modified with 1-aminomethylpyrene for the introduction of hydrophobic moieties, and were non-covalently complexed with PNG via p-p stacking and van der Waals forces. It resulted in the formation of PNG-AuNPCs that increased SERS intensity with an enhancement factor of 1.34 x 1011. The PNG induced a high degree of AuNP clustering by enhancing the non-covalent interactions between them, resulting in increased hotspot junctions at highly localized plasmonic centers. Furthermore, to show that the PNG-AuNPCs would serve as stable, reproducible, and highly sensitive SERS nanotags for biosensing, we formed sandwich-type immunocomplexes composed of the PNG-AuNPCs, immunoglobulin G (IgG) as the antigen, and magnetic beads. We found a linear relationship between SERS intensity and IgG concentration, with a limit of detection lower than 31.0 fM for IgG detection. Thus, the PNG-AuNPCs could be useful as SERS nanotags for highly sensitive SERS-based biosensing applications. | en_US |
dc.description.sponsorship | This work was supported by the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health and Welfare, Korea (HI15C2958), the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Korea (2015R1D1A1A01058026), the Ministry of Science and ICT, Korea (2008-0061891), and the Agency for Defense Development through Chemical and Biological Research Center, Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | REDUCED GRAPHENE OXIDE | en_US |
dc.subject | SILVER NANOPARTICLE | en_US |
dc.subject | GOLD NANOPARTICLES | en_US |
dc.subject | OPTICAL NANOPROBES | en_US |
dc.subject | SERS DETECTION | en_US |
dc.subject | DRUG-DELIVERY | en_US |
dc.subject | NANO-GRAPHENE | en_US |
dc.subject | CANCER | en_US |
dc.subject | SIZE | en_US |
dc.subject | SPECTROSCOPY | en_US |
dc.title | PEGylated nanographene-mediated metallic nanoparticle clusters for surface enhanced Raman scattering-based biosensing | en_US |
dc.type | Article | en_US |
dc.relation.no | 11 | - |
dc.relation.volume | 143 | - |
dc.identifier.doi | 10.1039/c8an00329g | - |
dc.relation.page | 2604-2615 | - |
dc.relation.journal | ANALYST | - |
dc.contributor.googleauthor | Ali, Ahmed | - |
dc.contributor.googleauthor | Hwang, Eun Young | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.contributor.googleauthor | Lim, Dong Woo | - |
dc.relation.code | 2018001992 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
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