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Morphological characterization and molecular phylogenetic analysis of Dolichospermum hangangense (Nostocales, Cyanobacteria) sp nov from Han River, Korea

Title
Morphological characterization and molecular phylogenetic analysis of Dolichospermum hangangense (Nostocales, Cyanobacteria) sp nov from Han River, Korea
Author
한명수
Keywords
Dolichospermum hangangense; morphology; rbcLX; 16S rRNA; 16S-23S ITS
Issue Date
2018-06
Publisher
KOREAN SOC PHYCOLOGY
Citation
ALGAE, v. 33, no. 2, page. 143-156
Abstract
Dolichospermum is a filamentous and heterocytous cyanobacterium that is one of the commonly occurring phytoplanktons in the Han River of Korea. Morphological observations led to the identification of D. planctonicum-like filaments in seasonal water samples. In the present study, we successfully isolated these filaments using culture methods, and examined its morphology using light and scanning electron microscopy. The morphology of the D. planctonicum-like species differed from that of typical D. planctonicum; it had thin cylindrical-shaped akinetes, which were narrower towards the ends than at the center. This morphology is firstly described in the genus Dolichospermum. In addition, the akinetes in the filament developed solitarily and were distant from the heterocytes. Phylogenetic analysis of the 16S rRNA sequences showed that our Dolichospermum clustered with D. planctonicum and D. circinale, which have coiled trichome. However, phylogenetic analysis of the gene encoding rivulose-1,5-bisphosphate carboxylase (rbcIA) clearly separated our species from other Dolichospermum, forming a unique Glade. Additionally, structures of D. planctonicum and D. hangangense strains were different type in Box-B and V3 region. These results demonstrated that the new Dolichospermum species was unique in morphology and molecular traits. Therefore, we propose this to be a new species belonging to genus Dolichospermum with the name Dolichospermu in hangangense sp. nov.
URI
https://www.e-algae.org/journal/view.php?doi=10.4490/algae.2018.33.5.2https://repository.hanyang.ac.kr/handle/20.500.11754/119045
ISSN
1226-2617; 2093-0860
DOI
10.4490/algae.2018.33.5.2
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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