Sensitive on-chip detection of cancer antigen 125 using a DNA aptamer/carbon nanotube network platform
- Title
- Sensitive on-chip detection of cancer antigen 125 using a DNA aptamer/carbon nanotube network platform
- Author
- 이해원
- Keywords
- Aptamer; CA125; MUC16; Ovarian cancer; CNT; Immunoassay
- Issue Date
- 2018-03
- Publisher
- ELSEVIER SCIENCE SA
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v. 256, page. 89-97
- Abstract
- Despite the major role of cancer antigen 125 (CA125) in cancer progression, its structural diversity makes antibody-based immunoassay of this protein difficult. Here we report the selection of an anti-CA125 ssDNA aptamer and its application to sensitive detection of CA125 when used together with a CA125-specific antibody immobilized on a three-dimensional network of carbon nanotubes (3DN-CNTs). With exceptional stability and easy modifications over antibodies, the selected ssDNA aptamer (rCAA-8) showed high binding affinity (166 nM) for recombinant CA125, which enabled CA125-specific imaging in ovarian cancer cells (OVCAR-3). Furthermore, when compared to other fluorescent assays based on graphene oxide or flat surface and traditional enzyme-linked immunosorbent assay, a chip-based assay using a 3DN-CNT surface and anti-CA125 antibody-aptamer pair resulted in higher sensitivity and broader dynamic range as a function of CA125 concentration, due to high target specificity and high surface loading density. Our on-chip aptamer-based assay will facilitate sensitive and specific monitoring of CA125 in the biological and clinical fields. (C) 2017 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0925400517319470?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/117992
- ISSN
- 0925-4005
- DOI
- 10.1016/j.snb.2017.10.049
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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