Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이수재 | - |
dc.date.accessioned | 2019-12-06T07:28:45Z | - |
dc.date.available | 2019-12-06T07:28:45Z | - |
dc.date.issued | 2018-03 | - |
dc.identifier.citation | MICROMACHINES, v. 9, no. 3, Article no. 106 | en_US |
dc.identifier.issn | 2072-666X | - |
dc.identifier.uri | https://www.mdpi.com/2072-666X/9/3/106 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117985 | - |
dc.description.abstract | Circulating tumor cells (CTCs) are regarded as a strong biomarker which includes clinically valuable information. However, CTCs are very rare and require precise separation and detection for effective clinical applications. Furthermore, downstream analysis has become necessary to identify the distinct sub-population of CTCs that causes metastasis. Here, we report a flow-restricted microfluidic trap array capable of deterministic single-cell capture of CTCs. The extent of flow restriction, correlating with the device geometry, was then optimized using a highly invasive breast cancer cell line (LM2 MDA-MB-231) to achieve 97% capture efficiency with a single-cell capture rate of 99%. Single-cell capture of CTCs from mice with full-blown metastasis was also demonstrated. The single-CTC capturing ability of the flow-restricted trap array not only showed cell enumerating ability but also high prospects for application in future automated downstream analysis. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (NRF-2017R1A2B4003189) and the Ministry of Education of Korea (NRF-2012R1A6A1029029). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI | en_US |
dc.subject | circulating tumor cell | en_US |
dc.subject | single-cell capture | en_US |
dc.subject | microfluidics | en_US |
dc.title | Deterministic Capture of Individual Circulating Tumor Cells Using a Flow-Restricted Microfluidic Trap Array | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 9 | - |
dc.identifier.doi | 10.3390/mi9030106 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | MICROMACHINES | - |
dc.contributor.googleauthor | Yoon, Yousang | - |
dc.contributor.googleauthor | Lee, Jusin | - |
dc.contributor.googleauthor | Yoo, Ki-Chun | - |
dc.contributor.googleauthor | Sul, Onejae | - |
dc.contributor.googleauthor | Lee, Su-Jae | - |
dc.contributor.googleauthor | Lee, Seung-Beck | - |
dc.relation.code | 2018009674 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | sj0420 | - |
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