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Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography

Title
Multispectral analog-mean-delay fluorescence lifetime imaging combined with optical coherence tomography
Author
유홍기
Keywords
NECROTIC CORE SIZE; ATHEROSCLEROTIC PLAQUES; INTRAVASCULAR ULTRASOUND; INDOCYANINE GREEN; CAP THICKNESS; FIBROUS CAP; LABEL-FREE; VIVO; MICROSCOPY; TISSUE
Issue Date
2018-03
Publisher
OPTICAL SOC AMER
Citation
BIOMEDICAL OPTICS EXPRESS, v. 9, no. 4, page. 1930-1947
Abstract
The pathophysiological progression of chronic diseases, including atherosclerosis and cancer, is closely related to compositional changes in biological tissues containing endogenous fluorophores such as collagen, elastin, and NADH, which exhibit strong autofluorescence under ultraviolet excitation. Fluorescence lifetime imaging (FLIm) provides robust detection of the compositional changes by measuring fluorescence lifetime, which is an inherent property of a fluorophore. In this paper, we present a dual-modality system combining a multispectral analog-mean-delay (AMD) FLIm and a high-speed swept-source optical coherence tomography (OCT) to simultaneously visualize the cross-sectional morphology and biochemical compositional information of a biological tissue. Experiments using standard fluorescent solutions showed that the fluorescence lifetime could be measured with a precision of less than 40 psec using the multispectral AMD-FLIm without averaging. In addition, we performed ex vivo imaging on rabbit iliac normal-looking and atherosclerotic specimens to demonstrate the feasibility of the combined FLIm-OCT system for atherosclerosis imaging. We expect that the combined FLIm-OCT will be a promising nextgeneration imaging technique for diagnosing atherosclerosis and cancer due to the advantages of the proposed label-free high-precision multispectral lifetime measurement. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
URI
https://www.osapublishing.org/boe/abstract.cfm?uri=boe-9-4-1930https://repository.hanyang.ac.kr/handle/20.500.11754/117818
ISSN
2156-7085
DOI
10.1364/BOE.9.001930
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > ELECTRICAL AND BIOMEDICAL ENGINEERING(전기·생체공학부) > Articles
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