Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 성기훈 | - |
dc.date.accessioned | 2019-12-05T16:11:30Z | - |
dc.date.available | 2019-12-05T16:11:30Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.citation | BIOMICROFLUIDICS, v. 12, no. 1, Article no. 014111 | en_US |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.uri | https://aip.scitation.org/doi/10.1063/1.5021395 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117627 | - |
dc.description.abstract | Self-monitoring of glycated albumin (GA), a useful glycemic marker, is an established method for preventing diabetes complications. Here, the paper-based lateral flow assay devices were developed for the sensitive detection of GA and the total human serum albumin (tHSA) in self-monitoring diabetes patients. Boronic acid-derived agarose beads were packed into a hole on a lateral flow channel. These well-coordinated agarose beads were used to capture GA through specific cis-diol interactions and to enhance the colorimetric signals by concentrating the target molecules. The devices exhibited large dynamic ranges (from 10 mu g/ml to 10 mg/ml for GA and from 10 mg/ml to 50 mg/ml for tHSA) and low detection limits (7.1 mu g/ml for GA and 4.7 mg/ml for tHSA), which cover the range of GA concentration in healthy plasma, which is 0.21-1.65 mg/ml (0.6%-3%). In determining the unknown GA concentrations in two commercial human plasma samples, the relative percentage difference between the values found by a standard ELISA kit and those found by our developed devices was 2.62% and 8.80%, which are within an acceptable range. The measurements of GA and tHSA were completed within 20 min for the total sample-to-answer diagnosis, fulfilling the demand for rapid analysis. Furthermore, the recovery values ranged from 99.4% to 110% in device accuracy tests. These results indicate that the developed paper-based device with boronic acid-derived agarose beads is a promising platform for GA and tHSA detection as applied to self-monitoring systems. Published by AIP Publishing. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2008-0061891). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER INST PHYSICS | en_US |
dc.subject | DIABETES-MELLITUS | en_US |
dc.subject | GLYCEMIC CONTROL | en_US |
dc.subject | MICROFLUIDICS | en_US |
dc.subject | HEMOGLOBIN | en_US |
dc.subject | GLUCOSE | en_US |
dc.subject | VALUES | en_US |
dc.title | Determination of glycated albumin using boronic acid-derived agarose beads on paper-based devices | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 12 | - |
dc.identifier.doi | 10.1063/1.5021395 | - |
dc.relation.page | 141111-141118 | - |
dc.relation.journal | BIOMICROFLUIDICS | - |
dc.contributor.googleauthor | Ko, Euna | - |
dc.contributor.googleauthor | Van-Khue Tran | - |
dc.contributor.googleauthor | Geng, Yanfang | - |
dc.contributor.googleauthor | Kim, Min Ki | - |
dc.contributor.googleauthor | Jin, Ga Hyun | - |
dc.contributor.googleauthor | Son, Seong Eun | - |
dc.contributor.googleauthor | Hur, Won | - |
dc.contributor.googleauthor | Seong, Gi Hun | - |
dc.relation.code | 2018000982 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | ghseong | - |
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