178 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author성기훈-
dc.date.accessioned2019-12-05T16:11:30Z-
dc.date.available2019-12-05T16:11:30Z-
dc.date.issued2018-02-
dc.identifier.citationBIOMICROFLUIDICS, v. 12, no. 1, Article no. 014111en_US
dc.identifier.issn1932-1058-
dc.identifier.urihttps://aip.scitation.org/doi/10.1063/1.5021395-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/117627-
dc.description.abstractSelf-monitoring of glycated albumin (GA), a useful glycemic marker, is an established method for preventing diabetes complications. Here, the paper-based lateral flow assay devices were developed for the sensitive detection of GA and the total human serum albumin (tHSA) in self-monitoring diabetes patients. Boronic acid-derived agarose beads were packed into a hole on a lateral flow channel. These well-coordinated agarose beads were used to capture GA through specific cis-diol interactions and to enhance the colorimetric signals by concentrating the target molecules. The devices exhibited large dynamic ranges (from 10 mu g/ml to 10 mg/ml for GA and from 10 mg/ml to 50 mg/ml for tHSA) and low detection limits (7.1 mu g/ml for GA and 4.7 mg/ml for tHSA), which cover the range of GA concentration in healthy plasma, which is 0.21-1.65 mg/ml (0.6%-3%). In determining the unknown GA concentrations in two commercial human plasma samples, the relative percentage difference between the values found by a standard ELISA kit and those found by our developed devices was 2.62% and 8.80%, which are within an acceptable range. The measurements of GA and tHSA were completed within 20 min for the total sample-to-answer diagnosis, fulfilling the demand for rapid analysis. Furthermore, the recovery values ranged from 99.4% to 110% in device accuracy tests. These results indicate that the developed paper-based device with boronic acid-derived agarose beads is a promising platform for GA and tHSA detection as applied to self-monitoring systems. Published by AIP Publishing.en_US
dc.description.sponsorshipThis research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2008-0061891).en_US
dc.language.isoen_USen_US
dc.publisherAMER INST PHYSICSen_US
dc.subjectDIABETES-MELLITUSen_US
dc.subjectGLYCEMIC CONTROLen_US
dc.subjectMICROFLUIDICSen_US
dc.subjectHEMOGLOBINen_US
dc.subjectGLUCOSEen_US
dc.subjectVALUESen_US
dc.titleDetermination of glycated albumin using boronic acid-derived agarose beads on paper-based devicesen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume12-
dc.identifier.doi10.1063/1.5021395-
dc.relation.page141111-141118-
dc.relation.journalBIOMICROFLUIDICS-
dc.contributor.googleauthorKo, Euna-
dc.contributor.googleauthorVan-Khue Tran-
dc.contributor.googleauthorGeng, Yanfang-
dc.contributor.googleauthorKim, Min Ki-
dc.contributor.googleauthorJin, Ga Hyun-
dc.contributor.googleauthorSon, Seong Eun-
dc.contributor.googleauthorHur, Won-
dc.contributor.googleauthorSeong, Gi Hun-
dc.relation.code2018000982-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidghseong-
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE