Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 황규태 | - |
dc.date.accessioned | 2019-12-04T06:09:14Z | - |
dc.date.available | 2019-12-04T06:09:14Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 495, no. 1, page. 1257-1263 | en_US |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0006291X1732332X?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117261 | - |
dc.description.abstract | This study was designed to identify and characterize primary bone-derived cells (BdCs) and investigate the potential role of osteoblast differentiation. Primary BdCs were isolated from surgical bone for comparative analysis with mesenchymal stem cells (MSCs) and fetal osteoblasts (FOBs) and for potential differentiation to mature osteoblasts. Using three different cells, we successfully cultivated human osteoblast differentiation and activity which were evaluated using microarray and biochemical methods. BdCs are more correlated to MSCs in bioinformatics result and similar with FOBs in gene expression. In particular, Osterix, osteoprogenitor marker, was high expressed in BdCs, while the expression in MSCs and FOBS were very low. Furthermore, BdCs exhibited a marked alkaline phosphatase (ALP) expression, early stage of osteogenic marker, and retained osteogenic properties and physiological changes into maturation as in FOBs. BdCs also showed an increase in bone morphogenic protein 2 (BMP2), osteopontin (OPN), and osteocalcin (OCN) mRNA expressions during differentiation. This study suggests that BdCs may be osteoprogenitor cells or undifferentiated preosteoblasts with strong capacity to differentiate toward mature osteoblasts. (C) 2017 Published by Elsevier Inc. | en_US |
dc.description.sponsorship | This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, & Future (2016R1A2B4008606), the Ministry of Education (2017R1A6A3A11034394), and the Ministry of Science, ICT and Future Planning (2017R1C1B5075203). It was also supported by a Korea Health Technology R&D grant through the Korea Health Industry Development Institute (KHIDI), which is funded by the Ministry of Health & Welfare, Republic of Korea (HI17C0888). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.subject | Human bone-derived cells | en_US |
dc.subject | Osteoprogenitor cells | en_US |
dc.subject | Undifferentiated preosteoblasts | en_US |
dc.subject | Osteoblasts differentiation | en_US |
dc.subject | Osteoblasts activity | en_US |
dc.title | Identification and characterization of human bone-derived cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 495 | - |
dc.identifier.doi | 10.1016/j.bbrc.2017.11.155 | - |
dc.relation.page | 1257-1263 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.googleauthor | Jo, Sungsin | - |
dc.contributor.googleauthor | Lee, Jin Kyu | - |
dc.contributor.googleauthor | Han, Jinil | - |
dc.contributor.googleauthor | Lee, Bitnara | - |
dc.contributor.googleauthor | Kang, Suman | - |
dc.contributor.googleauthor | Hwang, Kyu-Tae | - |
dc.contributor.googleauthor | Park, Ye-Soo | - |
dc.contributor.googleauthor | Kim, Tae-Hwan | - |
dc.relation.code | 2018001043 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hwangkt | - |
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