110 0

Nanoscale graphene oxide-induced metallic nanoparticle clustering for surface-enhanced Raman scattering-based IgG detection

Title
Nanoscale graphene oxide-induced metallic nanoparticle clustering for surface-enhanced Raman scattering-based IgG detection
Author
주재범
Keywords
Nanoscale graphene oxide; Metallic nanoparticles; Clusters; Surface enhanced Raman scattering; IgG detection
Issue Date
2018-01
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v. 255, pt. 1, page. 183-192, Part:1
Abstract
Surface-enhanced Raman scattering (SERS) has been of great interest to advance sensitive optical biosensors. Clustered metallic nanoparticles as SERS nanoprobes are important to detect specific biomolecules with ultra-sensitivity. We report herein that the cluster formation of gold nanoparticles (GNPs) was induced by nanoscale graphene oxides (NGOs) with 120 nm to develop a new class of SERS nanotags for biosensing. The GNPs with 4-mercaptopyridine as a Raman reporter were modified with 1-pyrenemethylamine to introduce hydrophobic moieties on the surfaces and were complexed with NGOs via noncovalent interactions of pi-pi stacking and van der Waals interactions, resulting in the formation of NGO-GNP clusters (NGO-GNPCs). The NGO-GNPCs increased the SERS signal 3- to 4-fold higher than that of the individual GNPs due to enhancement of the electromagnetic field at the interstices of the GNPs. As a proof of concept, the NGO-GNPCs as SERS nanoprobes and magnetic beads (MBs) were developed to detect immunoglobulin G (IgG). Sandwich-type immunocomplexes of the NGO-GNPCs, IgG, and MBs were formed, representing a linear correlation between Raman intensity and IgG concentration, and a limit of detection of 0.6 pM. It suggests that the NGO-GNPCs as SERS nanoprobes could open a new avenue for highly sensitive SERS-based biosensing. (C) 2017 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0925400517313552?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/117192
ISSN
0925-4005
DOI
10.1016/j.snb.2017.07.140
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE