Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 양철수 | - |
dc.date.accessioned | 2019-12-04T01:47:38Z | - |
dc.date.available | 2019-12-04T01:47:38Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | FRONTIERS IN IMMUNOLOGY, v. 9, Article no. 22 | en_US |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fimmu.2018.00022/full | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117021 | - |
dc.description.abstract | Macrophages are the prime innate immune cells of the inflammatory response, and the combination of multiple signaling inputs derived from the recognition of host factors [e.g., interferon-g (IFN-gamma)] and invading pathogen products (e.g., toll-like receptors (TLRs) agonists) are required to maintain essential macrophage function. The profound effects on biological outcomes of inflammation associated with IFN-gamma pretreatment ("priming") and TLR4 ligand bacterial lipopolysaccharide (LPS)-induced macrophage activation (M1 or classical activation) have long been recognized, but the underlying mechanisms are not well defined. Therefore, we analyzed gene expression profiles of macrophages and identified genes, transcription factors (TFs), and transcription co-factors (TcoFs) that are uniquely or highly expressed in IFN-gamma-mediated TLR4 ligand LPS-inducible versus only TLR4 ligand LPS-inducible primary macrophages. This macrophage gene expression has not been observed in macrophage cell lines. We also showed that interleukin (IL)-4 and IL-13 (M2 or alternative activation) elicited the induction of a distinct subset of genes related to M2 macrophage polarization. Importantly, this macrophage gene expression was also associated with promoter conservation. In particular, our approach revealed novel roles for the TFs and TcoFs in response to inflammation. We believe that the systematic approach presented herein is an important framework to better understand the transcriptional machinery of different macrophage subtypes. | en_US |
dc.description.sponsorship | This work was supported by a National Research Foundation of Korea (NRF) grant that was funded by the Korean government (MSIP) (2016R1D1A1B04934970 to KJ and 2011-0030049 and 2017R1A2B4012905 to YC). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | FRONTIERS MEDIA SA | en_US |
dc.subject | macrophages | en_US |
dc.subject | transcription factors | en_US |
dc.subject | interferon-g | en_US |
dc.subject | RNA-sequencing | en_US |
dc.subject | lipopolysaccharide | en_US |
dc.title | High-Resolution Mapping and Dynamics of the Transcriptome, Transcription Factors, and Transcription Co-Factor Networks in Classically and Alternatively Activated Macrophages | en_US |
dc.type | Article | en_US |
dc.relation.volume | 9 | - |
dc.identifier.doi | 10.3389/fimmu.2018.00022 | - |
dc.relation.page | 1-21 | - |
dc.relation.journal | FRONTIERS IN IMMUNOLOGY | - |
dc.contributor.googleauthor | Das, Amitabh | - |
dc.contributor.googleauthor | Yang, Chul-Su | - |
dc.contributor.googleauthor | Arifuzzaman, Sarder | - |
dc.contributor.googleauthor | Kim, Sojin | - |
dc.contributor.googleauthor | Kim, Sun Young | - |
dc.contributor.googleauthor | Jung, Kyoung Hwa | - |
dc.contributor.googleauthor | Lee, Young Seek | - |
dc.contributor.googleauthor | Chai, Young Gyu | - |
dc.relation.code | 2018006826 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | chulsuyang | - |
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