Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2019-12-04T00:59:56Z | - |
dc.date.available | 2019-12-04T00:59:56Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | BIOMATERIALS SCIENCE, v. 6, no. 2, page. 407-417 | en_US |
dc.identifier.issn | 2047-4830 | - |
dc.identifier.issn | 2047-4849 | - |
dc.identifier.uri | https://pubs.rsc.org/en/content/articlelanding/2018/BM/C7BM01088E#!divAbstract | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/116951 | - |
dc.description.abstract | A glioblastoma is a common primary brain tumor that expresses microRNA- 21 (miR-21), which inhibits the expression of pro-apoptotic genes such as phosphatase and tensin homologue (PTEN) and programmed cell death 4 (PDCD4). Therefore, an antisense-oligonucleotide against miR-21 (miR21ASO) could have therapeutic effects for glioblastomas. In this study, curcumin was loaded into deoxycholic acid-conjugated polyethylenimine (DP) micelles. The curcumin-loaded DP micelle (DP-Cur) was evaluated as a carrier for the combined delivery of curcumin and miR21ASO. Gel retardation and heparin competition assays showed that DP-Cur formed stable complexes with miR21ASO. The anti-tumor effects of the combined delivery of curcumin and miR21ASO were evaluated in C6 glioblastoma cells. In vitro transfection showed that DP-Cur had an miR21ASO delivery efficiency similar to that of polyethylenimine (25 kDa, PEI25k) and DP. In the C6 cells, the delivery of miR21ASO using DP-Cur effectively reduced the miR21 level. The miR21ASO/DP-Cur complex induced apoptosis more effectively than the single delivery of curcumin or miR21ASO. The therapeutic effect of the miR21ASO/DP-Cur complex was also evaluated in an intracranial glioblastoma animal model. The miR21ASO/DP-Cur complex reduced the tumor volume more effectively than single therapy of curcumin or miR21ASO. Immunohistochemistry showed that PDCD4 and PTEN were induced in the miR21ASO/DP and miR21ASO/DP-Cur complex groups. Therefore, DP-Cur is an efficient carrier of miR21ASO and the combined delivery of miR21ASO and curcumin may be useful in the development of combination therapy for glioblastoma. | en_US |
dc.description.sponsorship | This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03934778) and the Ministry of Science and ICT (NRF-2017R1A2B4009036). It was also supported by the Bio & Medical Technology Development Program through NRF funded by the Ministry of Science and ICT (NRF-2016M3A9B4918833). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | GENE-THERAPY | en_US |
dc.subject | HEPATOCELLULAR-CARCINOMA | en_US |
dc.subject | ENDOTHELIAL-CELLS | en_US |
dc.subject | PEPTIDE MICELLES | en_US |
dc.subject | HEME OXYGENASE-1 | en_US |
dc.subject | CANCER-THERAPY | en_US |
dc.subject | DELIVERY | en_US |
dc.subject | BRAIN | en_US |
dc.subject | APOPTOSIS | en_US |
dc.subject | OLIGODEOXYNUCLEOTIDE | en_US |
dc.title | A curcumin-loaded polymeric micelle as a carrier of a microRNA-21 antisense-oligonucleotide for enhanced anti-tumor effects in a glioblastoma animal model | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 6 | - |
dc.identifier.doi | 10.1039/c7bm01088e | - |
dc.relation.page | 407-417 | - |
dc.relation.journal | BIOMATERIALS SCIENCE | - |
dc.contributor.googleauthor | Tan, Xiaonan | - |
dc.contributor.googleauthor | Kim, Gyeungyun | - |
dc.contributor.googleauthor | Lee, Dahee | - |
dc.contributor.googleauthor | Oh, Jungju | - |
dc.contributor.googleauthor | Kim, Minkyung | - |
dc.contributor.googleauthor | Piao, Chunxian | - |
dc.contributor.googleauthor | Lee, Jaewon | - |
dc.contributor.googleauthor | Lee, Min Sang | - |
dc.contributor.googleauthor | Jeong, Ji Hoon | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2018005650 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.orcid | http://orcid.org/0000-0002-7083-9296 | - |
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