Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배재만 | - |
dc.date.accessioned | 2019-11-30T16:47:55Z | - |
dc.date.available | 2019-11-30T16:47:55Z | - |
dc.date.issued | 2017-09 | - |
dc.identifier.citation | EXPERIMENTAL ANIMALS, v. 66, no. 1, page. 29-39 | en_US |
dc.identifier.issn | 1341-1357 | - |
dc.identifier.issn | 1881-7122 | - |
dc.identifier.uri | https://www.jstage.jst.go.jp/article/expanim/66/1/66_16-0060/_article | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/115597 | - |
dc.description.abstract | We previously showed that prepubertal chronic caffeine exposure adversely affected the development of the testes in male rats. Here we investigated dose- and time-related effects of caffeine consumption on the testis throughout sexual maturation in prepubertal rats. A total of 80 male SD rats were randomly divided into four groups: controls and rats fed 20, 60, or 120 mg caffeine/kg/day, respectively, via gavage for 10, 20, 30, or 40 days. Preputial separation was monitored daily before the rats were sacrificed. Terminal blood samples were collected for hormone assay, and testes were grossly evaluated and weighed. One testis was processed for histological analysis, and the other was collected to isolate Leydig cells. Caffeine exposure significantly increased the relative weight of the testis in a dose-related manner after 30 days of exposure, whereas the absolute testis weight tended to decrease at the 120 mg dose of caffeine. The mean diameter of the seminiferous tubules and height of the germinal epithelium significantly decreased in the caffeine-fed groups after 40 days of caffeine exposure, which was accompanied by a reduced BrdU incorporation rate in germ cells. In addition, caffeine intake significantly reduced in vivo and ex vivo testosterone production in a dose-related manner. Our results demonstrate that caffeine exposure during sexual maturation alter the testicular microarchitecture and also slow germ cell proliferation even at the 20 mg dose level. Furthermore, caffeine may act directly on Leydig cells and interfere with testosterone production in a dose-related manner, consequently delaying onset of sexual maturation. | en_US |
dc.description.sponsorship | JB participated in design, data analysis, and development of the manuscript, HC and YC participated in the experiments and data analysis, and JR participated in the design of the study, data analysis, and supervision. JR takes responsibility for the integrity of the data analysis. All authors read and approved the final manuscript. This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education and Science (NRF-2014R1A1A2053601). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | INT PRESS EDITING CENTRE INC | en_US |
dc.subject | caffeine | en_US |
dc.subject | germ cell | en_US |
dc.subject | Leydig cell | en_US |
dc.subject | sexual maturation | en_US |
dc.subject | testosterone | en_US |
dc.title | Dose- and time-related effects of caffeine on the testis in immature male rats | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 66 | - |
dc.identifier.doi | 10.1538/expanim.16-0060 | - |
dc.relation.page | 29-39 | - |
dc.relation.journal | EXPERIMENTAL ANIMALS | - |
dc.contributor.googleauthor | Bae, Jaeman | - |
dc.contributor.googleauthor | Choi, Hyeonhae | - |
dc.contributor.googleauthor | Choi, Yuri | - |
dc.contributor.googleauthor | Roh, Jaesook | - |
dc.relation.code | 2017012589 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | obgybae | - |
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