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Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer

Title
Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer
Author
이수재
Keywords
Food poisoning; Cell-phage display; Polyvalant directed peptide polymer; Fluorescein isothiocyanate; Succinimidyl 3-(2-pyridyldithio)propionate; RT-PCR
Issue Date
2017-08
Publisher
SPRINGER WIEN
Citation
MICROCHIMICA ACTA, v. 184, no. 8, page. 2611-2620
Abstract
The authors describe three fluorescein-conjugated peptides generated by cell-phage display for use as a diagnostic probes for fluorescent detection and imaging of Salmonella enteritidis and Salmonella typhimurium. The authors also designed a polyvalent-directed peptide polymer synthesized with poly-D-lysine and bifunctional succinimidyl 3-(2pyridyldithio) propionate with an affinity and sensitivity that is higher by more than an order of magnitude compared to single peptides due to multiple binding site interactions. In order to establish a diagnostic system for food poisoning, imaging analysis was performed using fluorescence microscopy. The limit of detection of the diagnostic system based on polyvalent directed peptide interaction is 10(2) colony-forming units per mL for Salmonella.
URI
https://link.springer.com/article/10.1007%2Fs00604-017-2240-1https://repository.hanyang.ac.kr/handle/20.500.11754/115307
ISSN
0026-3672; 1436-5073
DOI
10.1007/s00604-017-2240-1
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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