Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyunsook Kim | - |
dc.date.accessioned | 2019-11-28T07:51:15Z | - |
dc.date.available | 2019-11-28T07:51:15Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.citation | FOOD CONTROL, v. 78, page. 436-442 | en_US |
dc.identifier.issn | 0956-7135 | - |
dc.identifier.issn | 1873-7129 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0956713517300762?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/115114 | - |
dc.description.abstract | Kefir is fermented milk produced by co-cultures of lactic and acetic acid bacteria and yeast that are encapsulated by an exopolysaccharide (EPS) called kefiran. Probiotic kefir provides many benefits, including antimicrobial effects against many pathogens. The mechanisms underlying these effects, however, are not yet completely understood. In this study, a total of 22 strains of lactic acid bacteria (LAB), including eight strains of Lactobacillus kefiranofaciens, two strains of Lactobacillus kefiri, seven strains of Lactococcus lactis, and five strains of Leuconostoc mesenteroides, were isolated from kefir and classified by 165 ribosomal RNA gene sequencing. The ability of each strain to produce EPS was determined, and Lactobacillus kefiranofaciens DN1 was found to have the highest EPS yield among all isolates. Lactobacillus kefiranofaciens DN1 produced EPS, using glucose and lactose, and EPS yield rose to 2.2 g/L in modified MRS broth (60 g/L glucose). The antimicrobial activities of the EPS produced by Lactobacillus kefiranofaciens DN1 (EPS_DN1) against Listeria monocytogenes and Salmonella Enteritidis were assessed by growth curve analysis. We found that EPS_DN1 at a concentration of at least 1% was able to exert bactericidal effects against both pathogens. Notably, results of high-performance size-exclusion chromatography (HPSEC) analysis indicated that EPS_DN1 was not kefiran, the major EPS of kefir grains, suggesting that EPS_DN1 represents a novel bioactive compound in kefir. (C) 2017 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) (grant number 2015R1A2A2A01005017) grant funded by the Korean government (MSIP: Ministry of Science, ICT and Future Planning). We acknowledge Hyun-Woo Lim for his contribution in this study. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Kefir | en_US |
dc.subject | Exopolysaccharide | en_US |
dc.subject | Lactobacillus kefiranofaciens | en_US |
dc.subject | Antimicrobial activity | en_US |
dc.subject | Listeria monocytogenes | en_US |
dc.subject | Salmonella Enteritidis | en_US |
dc.title | Characterization and antibacterial activity of a novel exopolysaccharide produced by Lactobacillus kefiranofaciens DN1 isolated from kefir | en_US |
dc.type | Article | en_US |
dc.relation.volume | 78 | - |
dc.identifier.doi | 10.1016/j.foodcont.2017.02.033 | - |
dc.relation.page | 436-442 | - |
dc.relation.journal | FOOD CONTROL | - |
dc.contributor.googleauthor | Jeong, Dana | - |
dc.contributor.googleauthor | Kim, Dong-Hyeon | - |
dc.contributor.googleauthor | Kang, Il-Byeong | - |
dc.contributor.googleauthor | Kim, Hyunsook | - |
dc.contributor.googleauthor | Song, Kwang-Young | - |
dc.contributor.googleauthor | Kim, Hong-Seok | - |
dc.contributor.googleauthor | Seo, Kun-Ho | - |
dc.relation.code | 2017000754 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF HUMAN ECOLOGY[S] | - |
dc.sector.department | DEPARTMENT OF FOOD & NUTRITION | - |
dc.identifier.pid | hyunsk15 | - |
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