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Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems

Title
Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems
Author
Jong Wook Hong
Keywords
FIELD-FLOW FRACTIONATION; EXOSOMES; VESICLES; MICROVESICLES; SIZE; QUANTIFICATION; DEVICE
Issue Date
2017-08
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v. 7, Article no. 9907
Abstract
Extracellular vesicles (EVs) are the cell-secreted nano-and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. The inherent complexity of EVs is a source of heterogeneity in various potential applications of the biological nanovesicles including analysis. To diminish heterogeneity, EV should be isolated and separated according to their sizes and cargos. However, current technologies do not meet the requirements. We showed noninvasive and precise separation of EVs based on their sizes without any recognizable damages. We separated atto-liter volumes of biological nanoparticles through operation of the present system showing relatively large volume of sample treatment to milliliters within an hour. We observed distinct size and morphological differences of 30 to 100 nm of exosomes and apoptotic bodies through TEM analysis. Indeed, we confirmed the biological moiety variations through immunoblotting with noninvasively separated EVs opening new windows in study and application of the biological nanoparticles.
URI
https://www.nature.com/articles/s41598-017-08826-whttps://repository.hanyang.ac.kr/handle/20.500.11754/115113
ISSN
2045-2322
DOI
10.1038/s41598-017-08826-w
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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