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Sorghum husk biomass as a potential substrate for production of cellulolytic and xylanolytic enzymes by Nocardiopsis sp KNU

Title
Sorghum husk biomass as a potential substrate for production of cellulolytic and xylanolytic enzymes by Nocardiopsis sp KNU
Author
Sanjay Prabhu Govindwar
Keywords
Nocardiopsis sp KNU; Rice husk; Sorghum husk; Alkali pretreatment; Enzymatic saccharification
Issue Date
2017-07
Publisher
SPRINGER HEIDELBERG
Citation
3 BIOTECH, v. 7, Article no. 163
Abstract
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme. Cellulase produced by Nocardiopsis sp. KNU utilizing sorghum husk as a substrate was found to retain its stability in various surfactants up to 90%. The produced enzyme was further tested for saccharification of mild alkali pre-treated rice husk. The changes in morphology and functional group were analyzed using scanning electron microscopy and Fourier transform infrared spectroscopy. Enzymatic saccharification confirmed the hydrolytic potential of crude cellulase. The hydrolysate products were analyzed by high-performance thin layer chromatography.
URI
https://link.springer.com/article/10.1007%2Fs13205-017-0800-zhttps://repository.hanyang.ac.kr/handle/20.500.11754/115011
ISSN
2190-572X; 2190-5738
DOI
10.1007/s13205-017-0800-z
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING(자원환경공학과) > Articles
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