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Graying the self-assembly of gold nanoparticles for improved enzyme activity assays

Title
Graying the self-assembly of gold nanoparticles for improved enzyme activity assays
Author
김영필
Keywords
Gold nanoparticle; Silver enhancement; Signal amplification; Self-assembly; Enzyme activity
Issue Date
2017-07
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v. 246, page. 271-277
Abstract
Despite the simplicity and usability of gold nanoparticle (AuNP)-based colorimetry in a large panel of bioassays, this technique still suffers from a poor detection limit. To overcome this issue, we report an improved AuNP-based colorimetric assay in combination with simple centrifugation and silver enhancement. As a proof-of-concept, two types of enzymes (i.e., protein phosphatase and protease) were constructed based on the self-assembly of thiol-stabilized AuNPs in the presence of peptides and metal ions. When the AuNP solutions were subjected to a short cycle of centrifugation and silver enhancement, the signal-to-background ratio (SBR) was distinctly improved by a factor of 5-10 in both enzyme activity assays, as compared to that of AuNP-based colorimetry. The detection limit achieved by using the silver enhancement was determined to be improved by a factor of 1.0-3.4. Furthermore, grayscale images of the silver enhancement allowed for a rapid andsimple enzyme assay that did not require measuring.the absorbance. Due to its ability to improve the detection senstivity in a facile way, we anticipate that this approach will be suitable for use in many AuNP colorimetric assays. (C) 2017 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0925400517302940?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/114953
ISSN
0925-4005
DOI
10.1016/j.snb.2017.02.067
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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