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Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells

Title
Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells
Author
박장환
Keywords
Direct conversion; Oligodendrocyte; iOPC; Efficiency
Issue Date
2017-07
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Citation
BIOMOLECULES & THERAPEUTICS, v. 25, no. 4, page. 362-366
Abstract
Direct conversion by trans-differentiation is of growing interest in cell therapy for incurable diseases. The efficiency of cell reprogramming and functionality of converted cells are important considerations in cell transplantation therapy. Here, we compared two representative protocols for the generation of Induced-oligodendrocyte progenitor cells (iOPCs) from mouse and rat fibroblasts. Then, we showed that induction of Nkx6.2, Olig2, and Sox10 (NOS) was more effective in mouse fibroblasts and that induction of Olig2, Sox10, and Zfp536 (OSZ) was more effective at reprogramming iOPCs from rat fibroblasts. However, OSZ-iOPCs did not show greater proliferation than NOS-induced cells. Because the efficiency of iOPCs generation appears to differ between cell species depending on transcription factors and culture conditions, it is important to select appropriate methods for efficient reprogramming.
URI
http://www.biomolther.org/journal/view.html?volume=25&number=4&spage=362&year=2017https://repository.hanyang.ac.kr/handle/20.500.11754/114949
ISSN
1976-9148; 2005-4483
DOI
10.4062/biomolther.2017.066
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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