Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells
- Title
- Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells
- Author
- 박장환
- Keywords
- Direct conversion; Oligodendrocyte; iOPC; Efficiency
- Issue Date
- 2017-07
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Citation
- BIOMOLECULES & THERAPEUTICS, v. 25, no. 4, page. 362-366
- Abstract
- Direct conversion by trans-differentiation is of growing interest in cell therapy for incurable diseases. The efficiency of cell reprogramming and functionality of converted cells are important considerations in cell transplantation therapy. Here, we compared two representative protocols for the generation of Induced-oligodendrocyte progenitor cells (iOPCs) from mouse and rat fibroblasts. Then, we showed that induction of Nkx6.2, Olig2, and Sox10 (NOS) was more effective in mouse fibroblasts and that induction of Olig2, Sox10, and Zfp536 (OSZ) was more effective at reprogramming iOPCs from rat fibroblasts. However, OSZ-iOPCs did not show greater proliferation than NOS-induced cells. Because the efficiency of iOPCs generation appears to differ between cell species depending on transcription factors and culture conditions, it is important to select appropriate methods for efficient reprogramming.
- URI
- http://www.biomolther.org/journal/view.html?volume=25&number=4&spage=362&year=2017https://repository.hanyang.ac.kr/handle/20.500.11754/114949
- ISSN
- 1976-9148; 2005-4483
- DOI
- 10.4062/biomolther.2017.066
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
- Comparison of Reprogramming Methods for Generation of Induced-Oligodendrocyte Precursor Cells.pdfDownload
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