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Two-stage label-free aptasensing platform for rapid detection of Cronobacter sakazakii in powdered infant formula

Title
Two-stage label-free aptasensing platform for rapid detection of Cronobacter sakazakii in powdered infant formula
Author
Hyunsook Kim
Keywords
Cronobacter sakazakii; Aptamer; Gold nanoparticles; Powdered infant formula
Issue Date
2017-07
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v. 239, page. 94-99
Abstract
Cronobacter sakazakii constitutes one of the most life-threatening foodborne pathogens in neonates, and is typically acquired via contaminated powdered infant formula. In this study, we developed a sensitive and convenient two-stage label-free aptasensing platform for colorimetric detection of C. sakazakii in powdered infant formula. In this system, C. sakazakii depletes aptamers from the test solution, and the reduction of aptamers induces aggregation of gold nanoparticles in salt, a process accompanied by a color change from red to purple. Under optimal conditions, C. sakazakii present in PIF at a concentration as low as 7.1 x 10(3) CFU mL(-1) could be visually detected within 30 min, with a linear range between 7.1 x 10(3) and 7.1 x 10(7) CFU mL(-1). This novel assay provides new opportunities to detect bacteria in real-world samples. (C) 2016 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0925400516312175?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/114880
ISSN
0925-4005
DOI
10.1016/j.snb.2016.07.173
Appears in Collections:
COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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