Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김용희 | - |
dc.date.accessioned | 2019-11-26T20:18:16Z | - |
dc.date.available | 2019-11-26T20:18:16Z | - |
dc.date.issued | 2017-07 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v. 262, page. 72-86 | en_US |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.issn | 1873-4995 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0168365917307289?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114813 | - |
dc.description.abstract | Endothelial dysfunction combined with inflammation leads to atherosclerosis. Endothelium-specific delivery of therapeutic agents at the cellular level-specifically in vivo-is still a difficult task for proper management of atherosclerosis. We designed a redox-sensitive poly(oligo-L-arginine) (rsPOLA) playing dual roles as an endothelium alpha-2 adrenoceptors(alpha-2ARs)-targeted gene carrier and as a substrate for endothelial nitric oxide synthase (eNOS). Overexpression of alpha-2ARs on atherosclerotic endothelial cells was confirmed and the eNOS/rsPOLA nanoplexes following systemic injection demonstrated to 1) enhance eNOS gene delivery into endothelial cells via alpha-2ARs/L-arginine specific binding, 2) increase intracellular level of nitric oxide, 3) suppress inflammatory response in endothelium and finally 4) reduce atherosclerotic plaque in a Ldlr(-/-) atherosclerotic mouse model. Among the tested nanoplexes [ eNOS/rsPOLA, eNOS/{poly(oligo-D-arginine), rsPODA} and eNOS/(racemic mixture, rsRM)], eNOS/rsPOLA reduced atherosclerotic inflammation most effectively as we hypothesized. Current treatment strategy provides strong potential for further development of a gene therapeutic system to ameliorate inflammation and progressive atherosclerotic plaques. | en_US |
dc.description.sponsorship | This work was partially supported by grants from the National Research Foundation of Korea (2014049587, 2015003019) and the Brain Korea 21 plus program (22A20130011095). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Redox-sensitive polymeric nanoparticles | en_US |
dc.subject | L-arginine | en_US |
dc.subject | Targeted delivery | en_US |
dc.subject | Endothelial nitric oxide synthase | en_US |
dc.subject | Inflammation | en_US |
dc.subject | Atherosclerosis | en_US |
dc.title | Amelioration of atherosclerotic inflammation and plaques via endothelial adrenoceptor-targeted eNOS gene delivery using redox-sensitive polymer bearing L-arginine | en_US |
dc.type | Article | en_US |
dc.relation.volume | 262 | - |
dc.identifier.doi | 10.1016/j.jconrel.2017.07.019 | - |
dc.relation.page | 72-86 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.contributor.googleauthor | Ain, Qurrat Ul | - |
dc.contributor.googleauthor | Chung, Hyunji | - |
dc.contributor.googleauthor | Chung, Jee Young | - |
dc.contributor.googleauthor | Choi, Jae-Hoon | - |
dc.contributor.googleauthor | Kim, Yong-Hee | - |
dc.relation.code | 2017003061 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | yongheekim | - |
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