Comprehensive analysis of human protein N-termini enables assessment of various protein forms
- Title
- Comprehensive analysis of human protein N-termini enables assessment of various protein forms
- Author
- 백은옥
- Keywords
- ALTERNATIVE TRANSLATION INITIATION; MS-GF PLUS; DATABASE; PROTEOMICS; PEPTIDES; ACETYLTRANSFERASES; IDENTIFICATION; SEARCH; ACETYLATION; INSIGHTS
- Issue Date
- 2017-07
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v. 7, Article no. 6599
- Abstract
- Various forms of protein (proteoforms) are generated by genetic variations, alternative splicing, alternative translation initiation, co- or post-translational modification and proteolysis. Different proteoforms are in part discovered by characterizing their N-terminal sequences. Here, we introduce an N-terminal-peptide-enrichment method, Nrich. Filter-aided negative selection formed the basis for the use of two N-blocking reagents and two endoproteases in this method. We identified 6,525 acetylated (or partially acetylated) and 6,570 free protein N-termini arising from 5,727 proteins in HEK293T human cells. The protein N-termini included translation initiation sites annotated in the UniProtKB database, putative alternative translational initiation sites, and N-terminal sites exposed after signal/transit/propeptide removal or unknown processing, revealing various proteoforms in cells. In addition, 46 novel protein N-termini were identified in 5' untranslated region (UTR) sequence with pseudo start codons. Our data showing the observation of N-terminal sequences of mature proteins constitutes a useful resource that may provide information for a better understanding of various proteoforms in cells.
- URI
- https://www.nature.com/articles/s41598-017-06314-9https://repository.hanyang.ac.kr/handle/20.500.11754/114765
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-017-06314-9
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > COMPUTER SCIENCE(컴퓨터소프트웨어학부) > Articles
- Files in This Item:
- Comprehensive analysis of human protein N-termini enables assessment of various protein forms.pdfDownload
- Export
- RIS (EndNote)
- XLS (Excel)
- XML