99 86

Development and characterization of heparin-immobilized polycaprolactone nanofibrous scaffolds for tissue engineering using gamma-irradiation

Title
Development and characterization of heparin-immobilized polycaprolactone nanofibrous scaffolds for tissue engineering using gamma-irradiation
Author
신흥수
Keywords
BONE MORPHOGENIC PROTEIN-2; GROWTH-FACTOR; COMPOSITE SCAFFOLDS; CALCIUM-PHOSPHATE; FIBROUS SCAFFOLD; ACRYLIC-ACID; STEM-CELLS; SURFACE; POLYMERIZATION; HYDROXYAPATITE
Issue Date
2017-06
Publisher
ROYAL SOC CHEMISTRY
Citation
RSC ADVANCES, v. 7, no. 15, page. 8963-8972
Abstract
Polycaprolactone (PCL) has been considered a useful material for orthopedic devices and osseous implants because of its biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces that reduce initial cell viability. In this study, we fabricated surface-modified PCL nanofibers for tissue engineering using radiation technology. We supplemented the hydrophilicity of the PCL nanofibers by introducing 2-aminoethyl methacrylate (AEMA) through gamma-irradiation and subsequently immobilized heparin onto the nanofibers using the EDC/NHS reaction. The SEM images show that there is almost no change in the morphology of nanofibers after radiation grafting of AEMA and heparin-immobilization onto PCL nanofibers. The surface properties of the scaffolds were characterized by ATR-FTIR, XPS, and fluorescamine staining in order to confirm the successful grafting of AEMA onto the PCL nanofibers. Immobilization of heparin was also confirmed by the amide I (1650 cm(-1)) and amide II group (1550 cm(-1)) from ATR-FTIR. The amounts of heparin were drastically increased on the AEMA-PCL nanofibers as revealed by TBO assay. The initial cell viability of hMSCs was significantly increased on the AEMA grafted nanofibers but grew slowly on heparin-immobilized nanofibers. The cumulative release of bone morphogenetic protein-2 (BMP-2) was slow and continuous onto the heparin-immobilized nanofibers (18.13 +/- 3.87 mu g mL(-1)) compared to PCL nanofibers (20.25 +/- 1.45 mu g mL(-1)). Therefore, heparin-immobilized nanofibers may be a good tool for tissue engineering applications using radiation technology.
URI
https://pubs.rsc.org/en/content/articlelanding/2017/RA/C6RA20082F#!divAbstracthttps://repository.hanyang.ac.kr/handle/20.500.11754/114613
ISSN
2046-2069
DOI
10.1039/c6ra20082f
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
Development and characterization of heparin-immobilized polycaprolactone nanofibrous scaffolds for tissue engineering using gamma-irradiation.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE