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DNA-free Genome Editing of Chlamydomonas reinhardtii Using CRISPR and Subsequent Mutant Analysis

Title
DNA-free Genome Editing of Chlamydomonas reinhardtii Using CRISPR and Subsequent Mutant Analysis
Author
진언선
Keywords
Genome editing; CRISPR-Cas9; Microalgae; Ribonucleoproteins; Chlamydomonas reinhardtii; DNA-free transformation
Issue Date
2017-06
Publisher
BIO-PROTOCOL
Citation
BIO-PROTOCOL, v. 7, no. 11, Article no. e2352
Abstract
We successfully introduced targeted knock-out of gene of interest in Chlamydomonas reinhardtii by using DNA-free CRISPR. In this protocol, the detailed procedures of an entire workflow cover from the initial target selection of CRISPR to the mutant analysis using next generation sequencing (NGS) technology. Furthermore, we introduce a web-based set of tools, named CRISPR RGEN tools (http://www.rgenome.net/), which provides all required tools from CRISPR target design to NGS data analysis.
URI
https://bio-protocol.org/e2352https://repository.hanyang.ac.kr/handle/20.500.11754/114514
ISSN
2331-8325
DOI
10.21769/BioProtoc.2352
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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