Tandem malonate-based glucosides (TMGs) for membrane protein structural studies
- Title
- Tandem malonate-based glucosides (TMGs) for membrane protein structural studies
- Author
- 채필석
- Keywords
- FACIAL AMPHIPHILES; MELIBIOSE PERMEASE; ESCHERICHIA-COLI; STABILIZATION; SOLUBILIZATION; DETERGENTS; CRYSTALLIZATION; BINDING; UAPA; MECHANISMS
- Issue Date
- 2017-06
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v. 7, Article no. 3963
- Abstract
- High-resolution membrane protein structures are essential for understanding the molecular basis of diverse biological events and important in drug development. Detergents are usually used to extract these bio-macromolecules from the membranes and maintain them in a soluble and stable state in aqueous solutions for downstream characterization. However, many eukaryotic membrane proteins solubilized in conventional detergents tend to undergo structural degradation, necessitating the development of new amphiphilic agents with enhanced properties. In this study, we designed and synthesized a novel class of glucoside amphiphiles, designated tandem malonate-based glucosides (TMGs). A few TMG agents proved effective at both stabilizing a range of membrane proteins and extracting proteins from the membrane environment. These favourable characteristics, along with synthetic convenience, indicate that these agents have potential in membrane protein research.
- URI
- https://www.nature.com/articles/s41598-017-03809-3https://repository.hanyang.ac.kr/handle/20.500.11754/114401
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-017-03809-3
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
- Files in This Item:
- Tandem malonate-based glucosides (TMGs) for membrane protein structural studies.pdfDownload
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