Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이승환 | - |
dc.date.accessioned | 2019-11-26T01:13:03Z | - |
dc.date.available | 2019-11-26T01:13:03Z | - |
dc.date.issued | 2017-06 | - |
dc.identifier.citation | ACS NANO, v. 11, no. 6, page. 5950-5959 | en_US |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.issn | 1936-086X | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acsnano.7b01722 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/114366 | - |
dc.description.abstract | The field-effect transistor (FET) has been used in the development of diagnostic tools for several decades, leading to high-performance biosensors. Therefore, the FET platform can provide the foundation for the next generation of analytical methods. A major role of G-protein-coupled receptors (GPCRs) is in the transfer of external signals into the cell and promoting human body functions; thus, their principle application is in the screening of new drugs. The research community uses efficient systems to screen potential GPCR drugs; nevertheless, the need to develop GPCR-conjugated analytical devices remains for next-generation new drug screening. In this study, we proposed an approach for studying receptor agonism and antagonism by combining the roles of FETs and GPCRs in a dopamine receptor D1 (DRD1)-conjugated FET system, which is a suitable substitute for conventional cell-based receptor assays. DRD1 was reconstituted and purified to mimic native binding pockets that have highly discriminative interactions with DRD1 agonists/antagonists. The real-time responses from the DRD1-nanohybrid FET were highly sensitive and selective for dopamine agonists/antagonists, and their maximal response levels were clearly different depending on their DRD1 affinities. Moreover, the equilibrium constants (K) were estimated by fitting the response levels. Each K value indicates the variation in the affinity between DRD1 and the agonists/antagonists; a greater K value corresponds to a stronger DRD1 affinity in agonism, whereas a lower K value in antagonism indicates a stronger dopamine-blocking effect. | en_US |
dc.description.sponsorship | This work was supported by the Brain Research Program through the National Research Foundation of Korea funded by the Ministry of Science; ICT & Future Planning (NRF-2016M3C7A1905384), a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), founded by the Ministry of Health & Welfare (H116C0950); and the KRIBB Initiative Research Program. The work was also supported by the Development Fund of Seoul National University funded by Dongjin Semichem. Co., Korea (0458-20130066), and the National Research Foundation funded by the Korean Government (MSIP) (No. NRF-2014R1A2A1A10053108). In addition, support was provided by the Korea Basic Science Institute (D37410). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | dopamine | en_US |
dc.subject | dopamine receptor D1 | en_US |
dc.subject | agonists-antagonists | en_US |
dc.subject | agonism-antagonism | en_US |
dc.subject | field-effect transistor | en_US |
dc.subject | nanohybrids | en_US |
dc.subject | equilibrium constants | en_US |
dc.title | Dopamine receptor D1 agonism and antagonism using a field-effect transistor assay | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1021/acsnano.7b01722 | - |
dc.relation.journal | ACS NANO | - |
dc.contributor.googleauthor | Park, Seon Joo | - |
dc.contributor.googleauthor | Yang, Heehong | - |
dc.contributor.googleauthor | Lee, Seung Hwan | - |
dc.contributor.googleauthor | Song, Hyun Seok | - |
dc.contributor.googleauthor | Park, Chul Soon | - |
dc.contributor.googleauthor | Bae, Joonwon | - |
dc.contributor.googleauthor | Kwon, Oh Seok | - |
dc.contributor.googleauthor | Park, Tai Hyun | - |
dc.contributor.googleauthor | Jang, Jyongsik | - |
dc.relation.code | 2017000564 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | vincero78 | - |
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