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gamma-Fe2O3@SiO2 core-shell structured nanoparticle: Fabrication via surface treatment and application for plasmid DNA purification

Title
gamma-Fe2O3@SiO2 core-shell structured nanoparticle: Fabrication via surface treatment and application for plasmid DNA purification
Author
최성철
Keywords
Maghemite; Surface treatment; Core-shell structure; Zeta potential; Plasmid DNA purification
Issue Date
2017-06
Publisher
ELSEVIER SCI LTD
Citation
CERAMICS INTERNATIONAL, v. 43, no. 15, page. 12888-12892
Abstract
Plasmid DNA was purified using core-shell gamma-Fe2O3@SiO2 nanoparticles synthesized by a common sol-gel method utilizing tetraethylorthosilicate (TEOS) as a silica precursor. The dispersibility and TEOS binding affinity of gamma-Fe2O3 nanoparticles were enhanced by acid/base treatment due to the formation of additional surface hydroxyl groups prior to SiO2 deposition. Since SiO2 was coated under basic conditions in the presence of ammonia as a catalyst, base-treated gamma-Fe2O3 nanoparticles were more suited for this modification due to exhibiting a larger zeta potential and a lower saturation magnetization loss. The performance of the synthesized gamma-Fe2O3@SiO2 nanoparticles with a 15-nm-thick SiO2 layer in the purification of plasmid DNA was compared to that of commercial Fe3O4-based magnetic beads. The used DNA samples exhibited similar purities, and all samples had similar theoretical plasmid DNA binding capacities. However, gamma-Fe2O3@SiO2 nanoparticles exhibited a faster separation speed and a larger saturation magnetization of 100 emu/g compared to that of commercial Fe3O4-based magnetic beads (75 emu/g).
URI
https://www.sciencedirect.com/science/article/pii/S0272884217313986?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/114356
ISSN
0272-8842; 1873-3956
DOI
10.1016/j.ceramint.2017.06.183
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > MATERIALS SCIENCE AND ENGINEERING(신소재공학부) > Articles
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