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Hydrogel-based three-dimensional cell culture for organ-on-a-chip applications

Title
Hydrogel-based three-dimensional cell culture for organ-on-a-chip applications
Author
이승환
Keywords
hydrogel; 3D cell culture; in vitro systems; organ-on-a-chip
Issue Date
2017-05
Publisher
WILEY
Citation
BIOTECHNOLOGY PROGRESS, v. 33, no. 3, page. 580-589
Abstract
Recent studies have reported that three-dimensionally cultured cells have more physiologically relevant functions than two-dimensionally cultured cells. Cells are three-dimensionally surrounded by the extracellular matrix (ECM) in complex in vivo microenvironments and interact with the ECM and neighboring cells. Therefore, replicating the ECM environment is key to the successful cell culture models. Various natural and synthetic hydrogels have been used to mimic ECM environments based on their physical, chemical, and biological characteristics, such as biocompatibility, biodegradability, and biochemical functional groups. Because of these characteristics, hydrogels have been combined with microtechnologies and used in organ-on-a-chip applications to more closely recapitulate the in vivo microenvironment. Therefore, appropriate hydrogels should be selected depending on the cell types and applications. The porosity of the selected hydrogel should be controlled to facilitate the movement of nutrients and oxygen. In this review, we describe various types of hydrogels, external stimulation-based gelation of hydrogels, and control of their porosity. Then, we introduce applications of hydrogels for organ-on-a-chip. Last, we also discuss the challenges of hydrogel-based three-dimensional cell culture techniques and propose future directions. (C) 2017 American Institute of Chemical Engineers.
URI
https://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2457https://repository.hanyang.ac.kr/handle/20.500.11754/114213
ISSN
8756-7938; 1520-6033
DOI
10.1002/btpr.2457
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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