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Feasibility of multiplexed gene mutation detection in plasma samples of colorectal cancer patients by mass spectrometric genotyping

Title
Feasibility of multiplexed gene mutation detection in plasma samples of colorectal cancer patients by mass spectrometric genotyping
Author
신수진
Keywords
CIRCULATING TUMOR DNA; ANTI-EGFR THERAPY; ACQUIRED-RESISTANCE; KRAS MUTATIONS; EVOLUTION; CETUXIMAB; CELLS; BRAF
Issue Date
2017-05
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v. 12, no. 5, Article no. e0176340
Abstract
Mutation analysis of circulating tumor DNA (ctDNA) has recently been introduced as a non-invasive tumor monitoring method. In this study, we tested the mass spectrometric-based MassARRAY platform for multiplexed gene mutation analysis of plasma samples from colorectal cancer (CRC) patients. A total of 160 patients, who underwent curative resection of either primary or metastatic CRC harboring KRAS mutations between 2005 and 2012, were included. Circulating DNA was isolated from plasma was analyzed on the MassARRAY platform with or without selective amplification of mutant DNA fragments. Tumor-specific KRAS mutations were detected in 39.6% (42/106) of patients with distant metastasis, and in 5.6% (3/54) of patients without distant metastasis. Selective amplification of the mutant allele increased sensitivity to 58.5% (62/106) for patients with distant metastasis, and 16.7% (9/54) for patients without distant metastasis. These mutation detection rates were no less than those of droplet digital polymerase chain reaction. Among patients with distant metastasis, detectable plasma KRAS mutations correlated with larger primary tumors and shorter overall survival rate (P = 0.014 and P = 0.003, respectively). In addition, activating PIK3CA mutations were detected together with KRAS mutations in two plasma samples. Taken together, massARRAY platform is a cost-effective, multigene mutation profiling technique for ctDNA with reasonable sensitivity and specificity.
URI
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0176340https://repository.hanyang.ac.kr/handle/20.500.11754/114198
ISSN
1932-6203
DOI
10.1371/journal.pone.0176340
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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