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Periostin-Binding DNA Aptamer Treatment Ameliorates Peritoneal Dialysis-Induced Peritoneal Fibrosis

Title
Periostin-Binding DNA Aptamer Treatment Ameliorates Peritoneal Dialysis-Induced Peritoneal Fibrosis
Author
권영은
Keywords
GROWTH-FACTOR-BETA; EPITHELIAL-MESENCHYMAL TRANSITION; MATRIX GENE-EXPRESSION; MESOTHELIAL CELLS; TGF-BETA; SIGNALING PATHWAY; DIABETIC MICE; RENAL-DISEASE; ANTIBODY; INFLAMMATION
Issue Date
2017-05
Publisher
CELL PRESS
Citation
MOLECULAR THERAPY-NUCLEIC ACIDS, v. 7, page. 396-407
Abstract
Peritoneal fibrosis is a major complication in peritoneal dialysis (PD) patients, which leads to dialysis discontinuation. Periostin, increased by transforming growth factor beta 1 (TGF-beta 1) stimulation, induces the expression of extracellular matrix (ECM) genes. Aberrant periostin expression has been demonstrated to be associated with PD-related peritoneal fibrosis. Therefore, the effect of periostin inhibition by an aptamer-based inhibitor on peritoneal fibrosis was evaluated. In vitro, TGF-beta 1 treatment upregulated periostin, fibronectin, alpha-smooth muscle actin (alpha-SMA), and Snail expression and reduced E-cadherin expression in human peritoneal mesothelial cells (HPMCs). Periostin small interfering RNA (siRNA) treatment ameliorated the TGF-beta 1-induced periostin, fibronectin, alpha-SMA, and Snail expression and restored E-cadherin expression in HPMCs. Similarly, the periostin-binding DNA aptamer (PA) also attenuated fibronectin, alpha-SMA, and Snail upregulation and E-cadherin downregulation in TGF-beta 1-stimulated HPMCs. In mice treated with PD solution for 4 weeks, the expression of periostin, fibronectin, alpha-SMA, and Snail was significantly increased in the peritoneum, whereas E-cadherin expression was significantly decreased. The thickness of the submesothelial layer and the intensity of Masson's trichrome staining in the PD group were significantly increased compared to the untreated group. These changes were significantly abrogated by the intraperitoneal administration of PA. These findings suggest that PA can be a potential therapeutic strategy for peritoneal fibrosis in PD patients.
URI
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(17)30171-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2162253117301713%3Fshowall%3Dtruehttps://repository.hanyang.ac.kr/handle/20.500.11754/114023
ISSN
2162-2531
DOI
10.1016/j.omtn.2017.05.001
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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