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SERS-based genetic assay for amplification-free detection of prostate cancer specific PCA3 mimic DNA

Title
SERS-based genetic assay for amplification-free detection of prostate cancer specific PCA3 mimic DNA
Author
주재범
Keywords
Prostate cancer antigen 3 (PCA3); Surface-enhanced Raman scattering; Amplification-free DNA detection; Hollow gold nanospheres; Polymerase chain reaction (PCR)
Issue Date
2017-05
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v. 251, page. 302-309
Abstract
We report the development of amplification-free surface-enhanced Raman scattering (SERS)-based DNA assays for the rapid and highly sensitive detection of prostate cancer antigen 3 (PCA3) mimic DNA. This technique does not require any DNA amplification process using thermo-cycles in conventional polymerase chain reaction (PCR) due to its highly sensitive detection capability. Herein, the PCA3 mimic DNA, composed of 45 nucleotide sequences, was sandwiched between two probe DNA-immobilized particles (ASO(735)-conjugated detection HGNs and ASO(683)-immobilized capture magnetic beads) by hybridization reactions. Its quantitative analysis was performed by monitoring the characteristic Raman peak intensity of sandwich DNA complexes. The limit of detection (LOD) is estimated to be 2.7 fM, which is about four orders of magnitude more sensitive than that of conventional PCR. This SERS-based DNA assay technique is expected to be a potentially useful tool for early disease diagnosis. (C) 2017 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0925400517308511?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/113996
ISSN
0925-4005
DOI
10.1016/j.snb.2017.05.039
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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