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Introduction of N-cadherin-binding motif to alginate hydrogels for controlled stem cell differentiation

Title
Introduction of N-cadherin-binding motif to alginate hydrogels for controlled stem cell differentiation
Author
이근용
Keywords
Cell-cell interaction; Low-density lipoprotein receptor-related protein 5; N-cadherin; Stem cell; Alginate hydrogel
Issue Date
2017-05
Publisher
ELSEVIER SCIENCE BV
Citation
COLLOIDS AND SURFACES B-BIOINTERFACES, v. 155, page. 229-237
Abstract
Control of stem cell fate and phenotype using biomimetic synthetic extracellular matrices (ECMs) is an important tissue engineering approach. Many studies have focused on improving cell-matrix interactions. However, proper control of cell-cell interactions using synthetic ECMs could be critical for tissue engineering, especially with undifferentiated stem cells. In this study, alginate hydrogels were modified with a peptide derived from the low-density lipoprotein receptor-related protein 5 (LRP5), which is known to bind to N-cadherin, as a cell-cell interaction motif. In vitro changes in the morphology and differentiation of mouse bone marrow stromal cells (D1 stem cells) cultured in LRP5-alginate hydrogels were investigated. LRP5-alginate gels successfully induced stem cell aggregation and enhanced chondrogenic differentiation of Dl stem cells, compared to RGD-alginate gels, at low cell density. This approach to tailoring synthetic biomimetic ECM5 using cell-cell interaction motifs may be critical in tissue engineering approaches using stem cells. (C) 2017 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0927776517302011?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/113938
ISSN
0927-7765; 1873-4367
DOI
10.1016/j.colsurfb.2017.04.014
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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