321 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author이상경-
dc.date.accessioned2019-11-24T18:12:10Z-
dc.date.available2019-11-24T18:12:10Z-
dc.date.issued2017-04-
dc.identifier.citationJOURNAL OF DRUG TARGETING, v. 25, no. 4, page. 320-329en_US
dc.identifier.issn1061-186X-
dc.identifier.issn1029-2330-
dc.identifier.urihttps://www.tandfonline.com/doi/abs/10.1080/1061186X.2016.1258566?journalCode=idrt20-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/113817-
dc.description.abstractsiRNA entrapment within endosomes is a significant problem encountered with siRNA delivery platforms that co-opt receptor-mediated entry pathways. Attachment of a cell-penetrating peptide (CPP), such as nona-arginine (9R) to a cell receptor-binding ligand like the Rabies virus glycoprotein, RVG, allows effective siRNA delivery to the cytoplasm by non-endocytic pathways, but a significant amount of siRNA complexes also enters the cell by ligand-induced receptor endocytosis and remain localized in endosomes. Here, we report that the incorporation of trileucine (3 Leu) residues as an endo-osmolytic moiety in the peptide improves endosomal escape and intracellular delivery of siRNA. The trileucine motif did not affect early non-endosomal mechanism of cytoplasmic siRNA delivery but enhanced target gene silencing by >20% only beyond 24 h of transfection when siRNA delivery is mostly through the endocytic route and siRNA trapped in the endosomes at later stages were subject to release into cytoplasm. The mechanism may involve endosomal membrane disruption as trileucine residues lysed RBCs selectively under endosomal pH conditions. Interestingly <3 Leu or >3 Leu residues were not as effective, suggesting that 3 Leu residues are useful for enhancing cytoplasmic delivery of siRNA routed through endosomes.en_US
dc.description.sponsorshipThis work was supported by Korea National Research Foundation [2009-0079989, 2014R1A1A2056664].en_US
dc.language.isoen_USen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.subjectsiRNA deliveryen_US
dc.subjecttrileucineen_US
dc.subjectendosomeen_US
dc.subjectligand-mediated endocytosisen_US
dc.titleTrileucine residues in a ligand-CPP-based siRNA delivery platform improve endosomal escape of siRNAen_US
dc.typeArticleen_US
dc.relation.no4-
dc.relation.volume25-
dc.identifier.doi10.1080/1061186X.2016.1258566-
dc.relation.page320-329-
dc.relation.journalJOURNAL OF DRUG TARGETING-
dc.contributor.googleauthorUllah, Irfan-
dc.contributor.googleauthorChung, Kunho-
dc.contributor.googleauthorBeloor, Jagadish-
dc.contributor.googleauthorKim, Jongkil-
dc.contributor.googleauthorCho, Minyoung-
dc.contributor.googleauthorKim, Nahyun-
dc.contributor.googleauthorLee, Kuen Yong-
dc.contributor.googleauthorKumar, Priti-
dc.contributor.googleauthorLee, Sang-Kyung-
dc.relation.code2017003251-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidsangkyunglee-
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE