Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김계성 | - |
dc.date.accessioned | 2019-11-24T14:45:33Z | - |
dc.date.available | 2019-11-24T14:45:33Z | - |
dc.date.issued | 2017-04 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, v. 49, Article no. e315 | en_US |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://www.nature.com/articles/emm20172 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/113702 | - |
dc.description.abstract | Spermatogonial stem cells (SSCs) are germline stem cells located along the basement membrane of seminiferous tubules in testes. Recently, SSCs were shown to be reprogrammed into multipotent SSCs (mSSCs). However, both the key factors and biological networks underlying this reprogramming remain elusive. Here, we present transcriptional regulatory networks (TRNs) that control cellular processes related to the SSC-to-mSSC reprogramming. Previously, we established intermediate SSCs (iSSCs) undergoing the transition to mSSCs and generated gene expression profiles of SSCs, iSSCs and mSSCs. By comparing these profiles, we identified 2643 genes that were up-regulated during the reprogramming process and 15 key transcription factors (TFs) that regulate these genes. Using the TF-target relationships, we developed TRNs describing how these TFs regulate three pluripotency-related processes (cell proliferation, stem cell maintenance and epigenetic regulation) during the reprogramming. The TRNs showed that 4 of the 15 TFs (Oct4/Pou5f1, Cux1, Zfp143 and E2f4) regulated cell proliferation during the early stages of reprogramming, whereas 11 TFs (Oct4/Pou5f1, Foxm1, Cux1, Zfp143, Trp53, E2f4, Esrrb, Nfyb, Nanog, Sox2 and Klf4) regulated the three pluripotency-related processes during the late stages of reprogramming. Our TRNs provide a model for the temporally coordinated transcriptional regulation of pluripotency-related processes during the SSC-to-mSSC reprogramming, which can be further tested in detailed functional studies. | en_US |
dc.description.sponsorship | This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (No. 2012M3A9B4028738) and the Institute for Basic Science (IBS-R013-G1-2014-a00) funded by the Korean Ministry of Science, ICT and Future Planning. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | TRANSFER-RNA SYNTHETASES | en_US |
dc.subject | PLURIPOTENCY | en_US |
dc.subject | TUMORIGENICITY | en_US |
dc.subject | GENERATION | en_US |
dc.subject | BIOLOGY | en_US |
dc.title | Transcriptional regulatory networks underlying the reprogramming of spermatogonial stem cells to multipotent stem cells | en_US |
dc.type | Article | en_US |
dc.relation.volume | 49 | - |
dc.identifier.doi | 10.1038/emm.2017.2 | - |
dc.relation.page | 1-11 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Jeong, Hoe-Su | - |
dc.contributor.googleauthor | Bhin, Jinhyuk | - |
dc.contributor.googleauthor | Kim, Hyung Joon | - |
dc.contributor.googleauthor | Hwang, Daehee | - |
dc.contributor.googleauthor | Lee, Dong Ryul | - |
dc.contributor.googleauthor | Kim, Kye-Seong | - |
dc.relation.code | 2017002500 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | ks66kim | - |
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