Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상훈 | - |
dc.date.accessioned | 2019-11-21T07:04:04Z | - |
dc.date.available | 2019-11-21T07:04:04Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE, v. 49, Article no. e300 | en_US |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://www.nature.com/articles/emm2016163 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/113254 | - |
dc.description.abstract | Developmental information aids stem cell biologists in producing tissue-specific cells. Recapitulation of the developmental profile of a specific cell type in an in vitro stem cell system provides a strategy for manipulating cell-fate choice during the differentiation process. Nurr1 and Foxa2 are potential candidates for genetic engineering to generate midbrain-type dopamine (DA) neurons for experimental and therapeutic applications in Parkinson's disease (PD), as forced expression of these genes in neural stem/precursor cells (NPCs) yields cells with a complete battery of midbrain DA neuron-specific genes. However, simple overexpression without considering their expression pattern in the developing midbrain tends to generate DA cells without adequate neuronal maturation and long-term maintenance of their phenotype in vitro and in vivo after transplantation. We here show that the physiological levels and timing of Nurr1 and Foxa2 expression can be replicated in NPCs by choosing the right vectors and promoters. Controlled expression combined with a strategy for transgene expression maintenance induced generation of fully mature midbrain-type DA neurons. These findings demonstrate the feasibility of cellular engineering for artificial cell-fate specification. | en_US |
dc.description.sponsorship | This work was supported by a grant from the Medical Research Center (2008-0062287), funded by the National Research Foundation of Korea (NRF) of the Ministry of Science, ICT and Future Planning, Republic of Korea to S-HL and by the Basic Science Research Program (NRF-2014R1A1A2057443) through the NRF funded by the Ministry of Education to J-wS. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | ORPHAN NUCLEAR RECEPTOR | en_US |
dc.subject | TYROSINE-HYDROXYLASE | en_US |
dc.subject | SUBSTANTIA-NIGRA | en_US |
dc.subject | GENE-EXPRESSION | en_US |
dc.subject | STEM-CELLS | en_US |
dc.subject | DIFFERENTIATION | en_US |
dc.subject | INDUCTION | en_US |
dc.subject | TRANSPLANTATION | en_US |
dc.subject | MAINTENANCE | en_US |
dc.subject | PRECURSORS | en_US |
dc.title | In vitro generation of mature midbrain-type dopamine neurons by adjusting exogenous Nurr1 and Foxa2 expressions to their physiologic patterns | en_US |
dc.type | Article | en_US |
dc.relation.volume | 49 | - |
dc.identifier.doi | 10.1038/emm.2016.163 | - |
dc.relation.page | 1-11 | - |
dc.relation.journal | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Kim, Taeho | - |
dc.contributor.googleauthor | Song, Jae-Jin | - |
dc.contributor.googleauthor | Puspita, Lesly | - |
dc.contributor.googleauthor | Valiulahi, Parvin | - |
dc.contributor.googleauthor | Shim, Jae-Won | - |
dc.contributor.googleauthor | Lee, Sang-Hun | - |
dc.relation.code | 2017002500 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | leesh | - |
dc.identifier.researcherID | Q-4650-2019 | - |
dc.identifier.orcid | http://orcid.org/0000-0002-9005-5966 | - |
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