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dc.contributor.author남진우-
dc.date.accessioned2019-11-21T05:32:18Z-
dc.date.available2019-11-21T05:32:18Z-
dc.date.issued2017-03-
dc.identifier.citationBMB REPORTSen_US
dc.identifier.issn1976-6696-
dc.identifier.issn1976-670X-
dc.identifier.urihttp://koreascience.or.kr/article/JAKO201716556450924.page-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/113165-
dc.description.abstractAt the post-transcriptional and translational levels, microRNA (miRNA) represses protein-coding genes via seed pairing to the 3' untranslated regions (UTRs) of mRNA. Although working models of miRNA-mediated gene silencing are successfully established using miRNA transfections and knockouts, the regulatory interaction between miRNA and long non-coding RNA (lncRNA) remain unknown. In particular, how the mRNA-resembling lncRNAs with 5' cap, 3' poly(A)-tail, or coding features, are regulated by miRNA is yet to be examined. We therefore investigated the functional interaction between miRNAs and lncRNAs with/without those features, in miRNA-transfected early zebrafish embryos. We observed that the greatest determinants of the miRNA-mediated silencing of lncRNAs were the 5' cap and 3' poly(A)-tails in lncRNAs, at both the post-transcriptional and translational levels. The lncRNAs confirmed to contain 5' cap, 3' poly(A)-tail, and the canonical miRNA target sites, were observed to be repressed in the level of both RNA and ribosome-protected fragment, while those with the miRNA target sites and without 5' cap and 3' poly(A)-tail, were not robustly repressed by miRNA introduction, thus suggesting a role as a miRNA-decoy.en_US
dc.description.sponsorshipWe thank all BIG Lab members for helpful discussions. This work was supported by the Basic Science Research Program through the National Research Foundation of Korea, and funded by the Ministry of Science, ICT & Future Planning (NRF-2012M3A9D1054516) and supported by the Program for Agriculture Science & Technology Development of the Rural Development Administration, Republic of Korea (Project No. PJ01045303).en_US
dc.language.isoen_USen_US
dc.publisherKOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 50, no. 4, page. 226-231en_US
dc.subjectlncRNAen_US
dc.subjectmiRNAen_US
dc.subjectsORFen_US
dc.subject3 ' poly(A)-tailen_US
dc.subject5 ' capen_US
dc.titlePost-transcriptional and translational regulation of mRNA-like long non-coding RNAs by microRNAs in early developmental stages of zebrafish embryosen_US
dc.typeArticleen_US
dc.relation.no4-
dc.relation.volume50-
dc.identifier.doi10.5483/BMBRep.2017.50.4.025-
dc.relation.page226-231-
dc.relation.journalBMB REPORTS-
dc.contributor.googleauthorLee, Kyung-Tae-
dc.contributor.googleauthorNam, Jin-Wu-
dc.relation.code2017010823-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidjwnam-


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