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Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells

Title
Transferrin-Conjugated Polymeric Nanoparticle for Receptor-Mediated Delivery of Doxorubicin in Doxorubicin-Resistant Breast Cancer Cells
Author
최한곤
Keywords
doxorubicin; doxorubicin-resistant cancer; polymeric nanoparticles; transferrin
Issue Date
2019-02
Publisher
MDPI
Citation
PHARMACEUTICS, v. 11, No. 2, Article no. 63
Abstract
In this study, a transferrin (T-f)-conjugated polymeric nanoparticle was developed for the targeted delivery of the chemotherapeutic agent doxorubicin (Dox) in order to overcome multi-drug resistance in cancer treatment. Our objective was to improve Dox delivery for producing significant antitumor efficacy in Dox-resistant (R) breast cancer cell lines with minimum toxicity to healthy cells. The results of our experiments revealed that Dox was successfully loaded inside a transferrin (T-f)-conjugated polymeric nanoparticle composed of poloxamer 407 (F127) and 123 (P123) (Dox/F127&P123-T-f), which produced nanosized particles (similar to 90 nm) with a low polydispersity index (similar to 0.23). The accelerated and controlled release profiles of Dox from the nanoparticles were characterized in acidic and physiological pH and Dox/F127&P123-T-f enhanced Dox cytotoxicity in OVCAR-3, MDA-MB-231, and MDA-MB-231(R) cell lines through induction of cellular apoptosis. Moreover, Dox/F127&P123-T-f inhibited cell migration and altered the cell cycle patterns of different cancer cells. In vivo study in MDA-MB-231(R) tumor-bearing mice demonstrated enhanced delivery of nanoparticles to the tumor site when coated in a targeting moiety. Therefore, Dox/F127&P123-T-f has been tailored, using the principles of nanotherapeutics, to overcome drug-resistant chemotherapy.
URI
https://www.mdpi.com/1999-4923/11/2/63https://repository.hanyang.ac.kr/handle/20.500.11754/112333
ISSN
1999-4923
DOI
10.3390/pharmaceutics11020063
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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