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Interaction-tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation

Title
Interaction-tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation
Author
이근용
Keywords
cell-matrix interaction; cell-cell interaction; RGD peptide; N-cadherin; alginate hydrogel
Issue Date
2017-01
Publisher
WILEY-BLACKWELL
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v. 105, no. 1, page. 42-50
Abstract
Controlling cell-matrix interactions is critical when transferring cells into the body using a scaffold, which can be elaborately tailored to successfully engineer the desired tissue. In this study, ATDC5 cells were encapsulated within alginate hydrogels and their chondrogenic differentiation was investigated in vitro. Cell-matrix interactions were introduced using RGD peptides, which improved the viability of encapsulated cells and enhanced the formation of condensed structures similar to a chondrogenic nodule. When N-cadherin of ATDC5 cells was blocked, the encapsulated cells did not form an aggregate, and chondrogenic differentiation could not be induced. Preformed cell aggregates with defined cell numbers in RGD-modified alginate gels retained adequate N-cadherin-mediated cell-cell interactions and increased chondrogenic marker gene expression, compared with the homogeneously dispersed cells in the gels. This approach may be useful to promote chondrogenesis with relatively few cells if they are encapsulated into a scaffold as a form of aggregates. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 42-50, 2017.
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.35865https://repository.hanyang.ac.kr/handle/20.500.11754/112276
ISSN
1549-3296; 1552-4965
DOI
10.1002/jbm.a.35865
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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