Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이용구 | - |
dc.date.accessioned | 2019-11-06T07:49:58Z | - |
dc.date.available | 2019-11-06T07:49:58Z | - |
dc.date.issued | 2019-04 | - |
dc.identifier.citation | DIABETES & METABOLISM JOURNAL, v. 43, no. e31, Page. 1-17 | en_US |
dc.identifier.issn | 2233-6079 | - |
dc.identifier.issn | 2233-6087 | - |
dc.identifier.uri | https://e-dmj.org/DOIx.php?id=10.4093/dmj.2018.0211 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/111943 | - |
dc.description.abstract | BACKGROUND: Recent studies have shown that microRNAs (miRNAs) are involved in the process of cardiomyocyte apoptosis. We have previously reported that granulocyte-colony stimulating factor (G-CSF) ameliorated diastolic dysfunction and attenuated cardiomyocyte apoptosis in a rat model of diabetic cardiomyopathy. In this study, we hypothesized a regulatory role of cardiac miRNAs in the mechanism of the anti-apoptotic effect of G-CSF in a diabetic cardiomyopathy rat model. METHODS: Rats were given a high-fat diet and low-dose streptozotocin injection and then randomly allocated to receive treatment with either G-CSF or saline. H9c2 rat cardiomyocytes were cultured under a high glucose (HG) condition to induce diabetic cardiomyopathy in vitro. We examined the extent of apoptosis, miRNA expression, and miRNA target genes in the myocardium and H9c2 cells. RESULTS: G-CSF treatment significantly decreased apoptosis and reduced miR-34a expression in diabetic myocardium and H9c2 cells under the HG condition. G-CSF treatment also significantly increased B-cell lymphoma 2 (Bcl-2) protein expression as a target for miR-34a. In addition, transfection with an miR-34a mimic significantly increased apoptosis and decreased Bcl-2 luciferase activity in H9c2 cells. CONCLUSION: Our results indicate that G-CSF might have an anti-apoptotic effect through down-regulation of miR-34a in a diabetic cardiomyopathy rat model. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1-A02062008 and 2016R1D1A1B03931479). | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN DIABETES ASSOC | en_US |
dc.subject | Diabetic cardiomyopathies | en_US |
dc.subject | Granulocyte colony-stimulating factor | en_US |
dc.subject | MicroRNAs | en_US |
dc.title | Role of MicroRNA-34a in Anti-Apoptotic Effects of Granulocyte-Colony Stimulating Factor in Diabetic Cardiomyopathy | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.4093/dmj.2018.0211 | - |
dc.relation.page | 1-17 | - |
dc.relation.journal | DIABETES & METABOLISM JOURNAL | - |
dc.contributor.googleauthor | Park, In-Hwa | - |
dc.contributor.googleauthor | Song, Yi-Sun | - |
dc.contributor.googleauthor | Joo, Hyun-Woo | - |
dc.contributor.googleauthor | Shen, Guang-Yin | - |
dc.contributor.googleauthor | Seong, Jin-Hee | - |
dc.contributor.googleauthor | Shin, Na-Kyoung | - |
dc.contributor.googleauthor | Cho, Young Jong | - |
dc.contributor.googleauthor | Lee, Yonggu | - |
dc.contributor.googleauthor | Shin, Jeong Hun | - |
dc.contributor.googleauthor | Lim, Young-Hyo | - |
dc.relation.code | 2019041673 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hmedi97 | - |
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