397 0

Chromatography Based Purification of rhGM-CSF from Recombinand Rice Cell Culture

Title
Chromatography Based Purification of rhGM-CSF from Recombinand Rice Cell Culture
Author
이은규
Issue Date
2005-11
Publisher
WILEY-V C H VERLAG GMBH
Citation
CHEMICAL ENGINEERING & TECHNOLOGY, v. 28, No. 11, Page. 1421-1425
Abstract
Plant cells are increasingly used as alternative host organisms for recombinant protein production requiring post-translational modifications because they have relatively low risks of hazardous viral contamination compared to animal cells. On the other hand, the plant cell culture route has intrinsic disadvantages, including a relatively low expression titer and difficulties in the purification. We developed a simple and scalable, chromatography based process to purify rhGM-CSF from recombinant rice cell culture. It consisted of cell removal by microfiltration, ammonium sulfate precipitation, anion exchange chromatography, and SEC-HPLC. alpha-Amylase, used as a leader sequence in the expression vector, was the major impurity protein co-purified with rhGM-CSF. The purification process resulted in a final purity of ca. 95% (as verified by size exclusion chromatography) and an overall recovery yield of ca. 48% from the culture broth (as verified by ELISA).
URI
https://onlinelibrary.wiley.com/doi/abs/10.1002/ceat.200500115https://repository.hanyang.ac.kr/handle/20.500.11754/111875
ISSN
0930-7516; 1521-4125
DOI
10.1002/ceat.200500115
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE